DNA initiation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 11.16 Å
EM Method: Single-particle
Fitted PDBs: 8v6g
Q-score: 0.054
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- Primase (Complex from Xenopus laevis)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Polymerase alpha (Complex from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna primer (2 kDa, RNA from synthetic construct)
DNA initiation complex (configuration 2) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 11.11 Å
EM Method: Single-particle
Fitted PDBs: 8v6h
Q-score: 0.018
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- Primase (Complex from Xenopus laevis)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Polymerase alpha (Complex from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna primer (2 kDa, RNA from synthetic construct)
DNA elongation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 14.06 Å
EM Method: Single-particle
Fitted PDBs: 8v6i
Q-score: 0.032
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Primase (Complex from Xenopus laevis)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Polymerase alpha (Complex from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- Rna-dna primer (6 kDa, Macromolecule from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna elongation substrate (20 kDa, Complex from synthetic construct)
- Polymerase alpha-primase with a dna elongation substrate (110 kDa, Complex)
DNA elongation complex (configuration 2) of Xenopus laevis DNA polymerase alpha-primase
Resolution: 11.11 Å
EM Method: Single-particle
Fitted PDBs: 8v6j
Q-score: 0.032
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Primase (Complex from Xenopus laevis)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Polymerase alpha (Complex from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- Rna-dna primer (6 kDa, Macromolecule from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna elongation substrate (20 kDa, Complex from synthetic construct)
- Polymerase alpha-primase with a dna elongation substrate (110 kDa, Complex)
Partial DNA termination subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8ucu
Q-score: 0.52
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase with a dna termination substrate (330 kDa, Complex)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna termination substrate (30 kDa, Complex from synthetic construct)
- Dna template (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Primase (110 kDa, Complex from Xenopus laevis)
- Rna-dna primer (9 kDa, DNA from synthetic construct)
Complete DNA termination subcomplex 1 of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 8ucv
Q-score: 0.337
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Rna-dna/dna primer (9 kDa, Macromolecule from synthetic construct)
- Polymerase alpha-primase with a dna termination substrate (330 kDa, Complex)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna termination substrate (30 kDa, Complex from synthetic construct)
- Dna template (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Primase (110 kDa, Complex from Xenopus laevis)
Complete DNA termination subcomplex 2 of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 8ucw
Q-score: 0.377
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Rna-dna/dna primer (9 kDa, Macromolecule from synthetic construct)
- Polymerase alpha-primase with a dna termination substrate (330 kDa, Complex)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna termination substrate (30 kDa, Complex from synthetic construct)
- Dna template (18 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Primase (110 kDa, Complex from Xenopus laevis)
Partial auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8g99
Q-score: 0.551
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Primase (Complex from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase (110 kDa, Complex)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
Complete auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8g9f
Q-score: 0.48
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Primase (Complex from Xenopus laevis)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase (110 kDa, Complex)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
DNA initiation subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8g9l
Q-score: 0.453
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ DOI: doi:10.1038/s41594-024-01227-4 Pubmed: 38491139 ]
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
- Primase (Complex from Xenopus laevis)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Polymerase alpha (Complex from Xenopus laevis)
- Dna template (15 kDa, DNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna primer (2 kDa, RNA from synthetic construct)