Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GpppAmU
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8jce
Q-score: 0.643
Law MCY, Zhang K, Tan YB, Nguyen TM, Luo D
J Biol Chem (2023) 299 pp. 105415-105415 [ DOI: doi:10.1016/j.jbc.2023.105415 Pubmed: 37918803 ]
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- Mrna-capping enzyme nsp1 (61 kDa, Protein from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- [(2~{r},3~{r},4~{r},5~{r})-5-(6-azanyl-7,8-dihydropurin-9-yl)-2-[[[[[(2~{r},3~{s},4~{r},5~{r})-5-(2-azanyl-7-methyl-6-oxidanylidene-1,8-dihydropurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-4-methoxy-oxolan-3-yl] [(2~{r},3~{s},4~{r},5~{s})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
GMPCPP-microtubule C1 reconstruction
Duan D, Lyu W, Chai P, Ma S, Wu K, Wu C, Xiong Y, Sestan N, Zhang K, Koleske AJ
Curr Biol (2023) 33 pp. 4582-4598.e10 [ DOI: doi:10.1016/j.cub.2023.09.018 Pubmed: 37858340 ]
- Gmpcpp-microtubules (500 kDa, Complex from Sus scrofa)
GMPCPP-microtubule decorated with Abl2-557-1090
Duan D, Lyu W, Chai P, Ma S, Wu K, Wu C, Xiong Y, Sestan N, Zhang K, Koleske AJ
Curr Biol (2023) 33 pp. 4582-4598.e10 [ DOI: doi:10.1016/j.cub.2023.09.018 Pubmed: 37858340 ]
- Abl2 c-terminals (557-1090) (Protein from Homo sapiens)
- Alpha-tubulin (Protein from Sus scrofa)
- Gmpcpp-microtubules decorated with abl2-557-1090. (500 kDa, Complex)
- Gmpcpp-microtubule (Complex from Sus scrofa)
- Abl2-557-1090 (Complex from Homo sapiens)
- Beta-tubulin (Protein from Sus scrofa)
Protofilament reconstruction of microtubule decorated with Abl2-557-1090 (consensus map)
Protofilament reconstruction of microtubule decorated with Abl2-557-1090, Class 1
Protofilament reconstruction of microtubule decorated with Abl2-557-1090, Class 2
Protofilament reconstruction of microtubule decorated with Abl2-557-1090, Class 3
rT66 Alt A-form (scaffold xover asymmetry)
Parsons M, Allan M, Li S, Shepherd T, Ratanalert S, Zhang K, Pullen K, Chiu W, Rouskin S, Bathe M
To Be Published
Parsons M, Allan M, Li S, Shepherd T, Ratanalert S, Zhang K, Pullen K, Chiu W, Rouskin S, Bathe M
To Be Published
- Rt66 hybrid-form (Complex from Inovirus M13)
Engineered human dynein motor domain in microtubule-bound state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8fcy
Q-score: 0.461
Ton WD, Wang Y, Chai P, Beauchamp-Perez C, Flint NT, Lammers LG, Xiong H, Zhang K, Markus SM
Nat Struct Mol Biol (2023) 30 pp. 1365-1379 [ Pubmed: 37322240 DOI: doi:10.1038/s41594-023-01010-x ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cytoplasmic dynein 1 heavy chain 1,serine--trna ligase (357 kDa, Protein from Homo sapiens)
- Engineered human dynein motor domain in microtubule-bound state (350 kDa, Complex from Homo sapiens)