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16-nm repeating structure of A-tubule from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
16-nm repeating structure of B-tubule from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
CryoET reconstruction of 48-nm repeat doublet microtubule from human sperm
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
48 nm-repeating structure of doublets from Tektin5-KO mouse sperm axoneme
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
48-nm repeating structure of doublets from mouse sperm flagella
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
- Dual specificity protein phosphatase 3 (20 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 161 (34 kDa, Protein from Mus musculus)
- Detyrosinated tubulin alpha-1a chain (50 kDa, Protein from Mus musculus)
- Tektin bundle-interacting protein 1 (23 kDa, Protein from Mus musculus)
- Nucleoside diphosphate kinase 7 (44 kDa, Protein from Mus musculus)
- Rib43a-like with coiled-coils protein 2 (45 kDa, Protein from Mus musculus)
- Sperm acrosome-associated protein 9 (19 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 45 (65 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member c2 (87 kDa, Protein from Mus musculus)
- Tektin-1 (48 kDa, Protein from Mus musculus)
- Enkurin (29 kDa, Protein from Mus musculus)
- Meiosis-specific nuclear structural protein 1 (60 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 2 protein (13 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 53 (62 kDa, Protein from Mus musculus)
- Tubulin beta-4b chain (49 kDa, Protein from Mus musculus)
- Sperm-associated antigen 8 (50 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 95 (26 kDa, Protein from Mus musculus)
- Tektin-2 (50 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 141 (12 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 52 (68 kDa, Protein from Mus musculus)
- Protein fam166b (30 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 276 (18 kDa, Protein from Mus musculus)
- Parkin coregulated gene protein homolog (27 kDa, Protein from Mus musculus)
- Protein flattop (20 kDa, Protein from Mus musculus)
- Ef-hand domain-containing protein 1 (75 kDa, Protein from Mus musculus)
- Tektin-4 (52 kDa, Protein from Mus musculus)
- Ef-hand domain-containing family member b (95 kDa, Protein from Mus musculus)
- Ef-hand calcium-binding domain-containing protein 6 (176 kDa, Protein from Mus musculus)
- Piercer of microtubule wall 1 protein (18 kDa, Protein from Mus musculus)
- Tektin-3 (56 kDa, Protein from Mus musculus)
- Tektin-5 (62 kDa, Protein from Mus musculus)
- Cilia- and flagella-associated protein 107 (23 kDa, Protein from Mus musculus)
- Mouse sperm (Cell from Mus musculus)
- Cilia- and flagella-associated protein 20 (22 kDa, Protein from Mus musculus)
- Coiled-coil domain-containing protein 105 (57 kDa, Protein from Mus musculus)
- Cilia- and flagella- associated protein 210 (65 kDa, Protein from Mus musculus)
Focused reconstruction of mouse doublet (register #1)
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
- Mouse sperm (Cell from Mus musculus)
Focused reconstruction of doublets from mouse sperm (register #2)
Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA
Cell (2023) 186 pp. 5041-5053.e19 [ DOI: doi:10.1016/j.cell.2023.09.017 Pubmed: 37865089 ]
- Mouse sperm (Cell from Mus musculus)
CryoET tilt series of mouse sperm flagella after FIB-SEM milling
Chen Z, Greenan GA, Shiozaki M, Liu Y, Skinner WM, Zhao X, Zhao S, Yan R, Yu Z, Lishko PV, Agard DA, Vale RD
Nat Struct Mol Biol (2023) [ DOI: 10.1038/s41594-022-00861-0 Pubmed: 36593309 ]
Image sets:
96-nm repeat unit of microtubule doublet from mouse sperm
Chen Z, Greenan GA, Shiozaki M, Liu Y, Skinner WM, Zhao X, Zhao S, Yan R, Yu Z, Lishko PV, Agard DA, Vale RD
Nat Struct Mol Biol (2023) 30 pp. 360-369 [ DOI: doi:10.1038/s41594-022-00861-0 Pubmed: 36593309 ]