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Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Phosphate ion (94 Da, Ligand)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin filament (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phalloidin. (Complex from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Cryo-EM reconstruction of the formin Cdc12 bound to the barbed end of F-actin (without phalloidin)
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- S. pombe cdc12 (Protein from Schizosaccharomyces pombe)
- Actin filament (Complex)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of f-actin. (Complex from Homo sapiens)
- Human cytoplasmic beta-actin (Protein from Homo sapiens)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Phosphate ion (94 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Phalloidin (Complex from Amanita phalloides)
Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Phosphate ion (94 Da, Ligand)
- Actin filament (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
Structure of the F-actin barbed end bound by formin mDia1
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8ru2
Q-score: 0.362
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Mdia1-bound f-actin barbed end. (Complex)
- Methylated-dna--protein-cysteine methyltransferase,protein diaphanous homolog 1 (86 kDa, Protein from Mus musculus)
- Mouse mdia1 (fh1fh2c domain) (Complex from Mus musculus)
- Actin filament (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Phosphate ion (94 Da, Ligand)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Inf2 dimer (Complex from Homo sapiens)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the R183W mutation
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 8oi8
Q-score: 0.679
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Actin filament harboring the r183w mutation. (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of the undecorated barbed end of filamentous beta/gamma actin
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8oi6
Q-score: 0.464
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Cytosolic beta-gamma actin (Complex from Bos taurus)
- Phalloidin (Complex from Amanita phalloides)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bos taurus)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin-phalloidin complex (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the N111S mutation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8oid
Q-score: 0.681
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin filament harboring the n111s mutation. (Complex from Homo sapiens)
Cryo-EM structure of F-actin in the Mg2+-ADP-BeF3- nucleotide state.
Resolution: 2.17 Å
EM Method: Single-particle
Fitted PDBs: 8a2r
Q-score: 0.692
Oosterheert W, Klink BU, Belyy A, Pospich S, Raunser S
Nature (2022) 611 pp. 374-379 [ Pubmed: 36289337 DOI: doi:10.1038/s41586-022-05241-8 ]
- Actin, alpha skeletal muscle (41 kDa, Protein from rabbit)
- Rabbit skeletal alpha-actin in the filamentous state. (15 MDa, Complex from Oryctolagus cuniculus)
- Beryllium trifluoride ion (66 Da, Ligand)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)