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CryoEM Structure of E. coli Transcription-Coupled Ribonucleotide Excision Repair (TC-RER) complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7uwe
Q-score: 0.413
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ DOI: doi:10.1016/j.cell.2023.04.029 Pubmed: 37196657 ]
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Elongation complex(ec)-rnaseh2 complex (Complex from Escherichia coli)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7uwh
Q-score: 0.458
Hao Z, Gowder M, Proshkin S, Bharati BK, Epshtein V, Svetlov V, Shamovsky I, Nudler E
Cell (2023) 186 pp. 2425- [ DOI: doi:10.1016/j.cell.2023.04.029 Pubmed: 37196657 ]
- Elongation complex(ec)-rnaseh2 complex bound to ribonucleotide substrate (Complex from Escherichia coli)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli)
- Dna/rna (59-mer) (18 kDa, Macromolecule from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna (59-mer) (18 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Ribonuclease hii (21 kDa, Protein from Escherichia coli)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8if8
Q-score: 0.552
Gong Y, Wei C, Wang J, Mu N, Lu Q, Wu C, Yan N, Yang H, Zhao Y, Yang X, Gurcha SS, Veerapen N, Batt SM, Hao Z, Da L, Besra GS, Rao Z, Zhang L
PNAS (2023) 120 pp. e2302858120-e2302858120 [ DOI: doi:10.1073/pnas.2302858120 Pubmed: 37252995 ]
- Calcium ion (40 Da, Ligand)
- Afta dimer (69 kDa, Complex from Mycobacterium tuberculosis H37Rv)
- Galactan 5-o-arabinofuranosyltransferase (69 kDa, Protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv))
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6xas
Q-score: 0.334
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ Pubmed: 33296676 DOI: doi:10.1016/j.molcel.2020.11.013 ]
- Rna (60-mer) (19 kDa, RNA from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli (strain K12))
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli (strain K12))
- Dna (29-mer) (8 kDa, DNA from Escherichia coli (strain K12))
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG
Resolution: 7.7 Å
EM Method: Single-particle
Fitted PDBs: 6xav
Q-score: 0.103
Hao Z, Epshtein V, Kim KH, Proshkin S, Svetlov V, Kamarthapu V, Bharati B, Mironov A, Walz T, Nudler E
Mol Cell (2021) 81 pp. 281- [ Pubmed: 33296676 DOI: doi:10.1016/j.molcel.2020.11.013 ]
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli K-12)
- Dna (29-mer) (9 kDa, DNA from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- Rna (18-mer) (5 kDa, RNA from Escherichia coli K-12)
- Dna (29-mer) (8 kDa, DNA from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- E. coli rho-dependent transcription pre-termination complex (726 kDa, Complex from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)