ATP-bound AMP-activated protein kinase
Resolution: 3.93 Å
EM Method: Single-particle
Fitted PDBs: 7m74
Q-score: 0.27
Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K
Science (2021) 373 pp. 413-419 [ DOI: doi:10.1126/science.abe7565 Pubmed: 34437114 ]
- 5'-amp-activated protein kinase subunit beta-2 (22 kDa, Protein from Homo sapiens)
- Adenosine monophosphate (347 Da, Ligand)
- Fab light chain (22 kDa, Protein from Homo sapiens)
- Nanobody (13 kDa, Protein from Lama glama)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fab heavy chain (24 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 5'-amp-activated protein kinase subunit gamma-1 (34 kDa, Protein from Homo sapiens)
- 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine (399 Da, Ligand)
- Mbp-fused atp bound ampk in complex with c-compound stabilized by fab and a nanobody (Complex from Homo sapiens)
- Maltose/maltodextrin abc transporter substrate-binding protein male (40 kDa, Protein from Escherichia coli)
- 5'-amp-activated protein kinase catalytic subunit alpha-1 (56 kDa, Protein from Homo sapiens)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7jhg
Q-score: 0.345
Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K
Science (2021) 373 pp. 413-419 [ DOI: doi:10.1126/science.abe7565 Pubmed: 34437114 ]
- 5'-amp-activated protein kinase subunit beta-2 (22 kDa, Protein from Homo sapiens)
- Fab light chain (23 kDa, Protein from synthetic construct)
- Adenosine monophosphate (347 Da, Ligand)
- Atp-bound fully inactive ampk in complex with dorsomorphin (compound c) (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fab heavy chain (25 kDa, Protein from synthetic construct)
- Fab-nanobody (Complex from synthetic construct)
- Nanobody (17 kDa, Protein from synthetic construct)
- Cryo-em structure of atp-bound fully inactive ampk in complex with dorsomorphin (compound c) and fab-nanobody (220 kDa, Complex)
- 5'-amp-activated protein kinase subunit gamma-1 (34 kDa, Protein from Homo sapiens)
- 6-[4-(2-piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine (399 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 5'-amp-activated protein kinase catalytic subunit alpha-1 (56 kDa, Protein from Homo sapiens)
- Maltodextrin-binding protein (40 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Fab and nanobody
Resolution: 3.92 Å
EM Method: Single-particle
Fitted PDBs: 7jhh
Q-score: 0.279
Yan Y, Mukherjee S, Harikumar KG, Strutzenberg TS, Zhou XE, Suino-Powell K, Xu TH, Sheldon RD, Lamp J, Brunzelle JS, Radziwon K, Ellis A, Novick SJ, Vega IE, Jones RG, Miller LJ, Xu HE, Griffin PR, Kossiakoff AA, Melcher K
Science (2021) 373 pp. 413-419 [ DOI: doi:10.1126/science.abe7565 Pubmed: 34437114 ]
- 5'-amp-activated protein kinase subunit beta-2 (22 kDa, Protein from Homo sapiens)
- Fab light chain (23 kDa, Protein from synthetic construct)
- Adenosine monophosphate (347 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Fab heavy chain (25 kDa, Protein from synthetic construct)
- Ampk complex with fab and nanobody (220 kDa, Complex)
- Nanobody (17 kDa, Protein from synthetic construct)
- Ampk (Complex from Homo sapiens)
- 5'-amp-activated protein kinase subunit gamma-1 (34 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 5'-amp-activated protein kinase catalytic subunit alpha-1 (56 kDa, Protein from Homo sapiens)
- Fab, nanobody (Complex from synthetic construct)
- Maltodextrin-binding protein (40 kDa, Protein from Escherichia coli K-12)