Cryo-EM structure of human TFIIIC bound and unbound to tRNA gene
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Müller CW
Sci Adv (2023) 9 [ Pubmed: 37418517 DOI: 10.1126/sciadv.adh2019 ]
Image sets:
- Intermediate particles of TauB-dimer subcomplex for mapping pair distance
- Final particles of TauB-dimer subcomplex (No DNA sample)
- Unaligned multi-frame micrographs of human TFIIIC bound to tRNA gene
- Final particles of TauA subcomplex (No DNA sample)
- Final particles of TauB-dimer subcomplex
- Unaligned multi-frame micrographs of human TFIIIC (No DNA sample)
- Final particles of TauA subcomplex
Local refinement map of TFIIIC TauB-DNA monomer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cli
Q-score: 0.492
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taub-dna monomer (300 kDa, Complex from Homo sapiens)
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
- Dna (35-mer) (10 kDa, DNA from Homo sapiens)
- Dna (35-mer) (10 kDa, DNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8clj
Q-score: 0.49
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tdna (35-mer) (10 kDa, DNA from Homo sapiens)
- Tfiiic taub-dna dimer (600 kDa, Complex from Homo sapiens)
- Tdna (35-mer) (10 kDa, DNA from Homo sapiens)
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8clk
Q-score: 0.358
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taua subcomplex (330 kDa, Complex from Homo sapiens)
- General transcription factor 3c polypeptide 3 (101 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 5 (61 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 6 (24 kDa, Protein from Homo sapiens)
TFIIIC TauA complex map (sample without DNA)
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taua subcomplex (330 kDa, Complex from Homo sapiens)
Structural insights into human TFIIIC promoter recognition
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8cll
Q-score: 0.445
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- General transcription factor 3c polypeptide 2 (102 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 4 (92 kDa, Protein from Homo sapiens)
- General transcription factor 3c polypeptide 1 (244 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Tfiiic taub dimer (560 kDa, Complex from Homo sapiens)
Consensus map of TauB-DNA dimer (Nu-refinement)
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taub-dna dimer (600 kDa, Complex from Homo sapiens)
Local refinement map of TFIIIC TauB-DNA monomer 2
Seifert-Davila W, Girbig M, Hauptmann L, Hoffmann T, Eustermann S, Muller CW
Sci Adv (2023) 9 pp. eadh2019-eadh2019 [ Pubmed: 37418517 DOI: doi:10.1126/sciadv.adh2019 ]
- Tfiiic taub-dna monomer 2 (600 kDa, Complex from Homo sapiens)
Structure of yeast RNA Polymerase III Pre-Termination Complex (PTC)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7z1l
Q-score: 0.538
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ Pubmed: 36070694 DOI: doi:10.1016/j.celrep.2022.111316 ]
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (7 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ptc (730 kDa, Complex from Saccharomyces cerevisiae W303)
Structure of yeast RNA Polymerase III Elongation Complex (EC)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z1m
Q-score: 0.466
Girbig M, Xie J, Grotsch H, Libri D, Porrua O, Muller CW
Cell Rep (2022) 40 pp. 111316-111316 [ Pubmed: 36070694 DOI: doi:10.1016/j.celrep.2022.111316 ]
- Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae W303)
- Nt-dna (13 kDa, Macromolecule from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast rna polymerase iii ec (730 kDa, Complex from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae W303)
- T-dna (13 kDa, Macromolecule from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna (6 kDa, RNA from synthetic construct)