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Structural insights into the binding of bS1 to the ribosome
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 8a3l
Q-score: 0.46
D'Urso G, Chat S, Gillet R, Giudice E
Nucleic Acids Res (2023) 51 pp. 3410-3419 [ Pubmed: 36840711 DOI: doi:10.1093/nar/gkad126 ]
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Elongation competent ribosome in complex with bs1 (2 GDa, Complex from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- P-site fmet-trna(fmet) (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- Mrna (12 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- E-site trna (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
21S ribosomal precursors induced by heat shock.
Giudice E, Georgeault S, Lavigne R, Pineau C, Trautwetter A, Ermel G, Blanco C, Gillet R
Int J Mol Sci (2023) 24 [ DOI: doi:10.3390/ijms24043491 Pubmed: 36834906 ]
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 7nbu
Q-score: 0.521
Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D'urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P
Nat Commun (2022) 13 pp. 2641-2641 [ Pubmed: 35552387 DOI: doi:10.1038/s41467-022-30373-w ]
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- Cspa mrna (3 kDa, RNA from Mycobacterium tuberculosis H37Rv)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- P-site fmet-trna(fmet) (Complex from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Higb1 toxin (Complex from Mycobacterium tuberculosis)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal subunit (Complex from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- Cspa mrna (Complex from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- E-site trna (589 Da, RNA from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- P-site fmet-trna(fmet) (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- E-site trna (Complex from Escherichia coli K-12)
- 50s ribosomal protein l16 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 16s rrna (Complex from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal proteins (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l16,50s ribosomal protein l31 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal proteins (Complex from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- M. tuberculosis higb1_k95a mutant toxin and its target, the cspa mrna, on the e. coli ribosome. (Complex from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 23s and 5s rrna (Complex from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- Probable endoribonuclease higb1 (14 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Structure of pre-accomodated trans-translation complex on E. coli stalled ribosome.
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7abz
Q-score: 0.359
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
Nat Commun (2021) 12 pp. 4909-4909 [ DOI: doi:10.1038/s41467-021-24881-4 Pubmed: 34389707 ]
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- Mrna (4 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- Transfer-messenger rna (tmrna) (117 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- Kirromycin (796 Da, Ligand)
- Transfer-messenger rna (tmrna) (Complex from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- Structure of pre-accomodated trans-translation complex on e. coli stalled ribosome. (Complex)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Elongation factor tu 2 (43 kDa, Protein from Escherichia coli K-12)
- Trna-phe and mrna (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- Trna-phe (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l10 (13 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein and elongation factor tu 2 (Complex from Escherichia coli (strain K12))
Structure of accomodated trans-translation complex on E. Coli stalled ribosome.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7ac7
Q-score: 0.478
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
Nat Commun (2021) 12 pp. 4909-4909 [ DOI: doi:10.1038/s41467-021-24881-4 Pubmed: 34389707 ]
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- Structure of accomodated trans-translation complex on e. coli stalled ribosome. (Complex)
- Transfer-messenger rna (tmrna) (117 kDa, RNA from Escherichia coli)
- Tmrna (Complex from Escherichia coli)
- Mrna (4 kDa, RNA from Escherichia coli)
- Trna-phe (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- Nascent peptide (236 Da, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- Trna, mrna (Complex from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (Complex from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l10 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
Structure of translocated trans-translation complex on E. coli stalled ribosome.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7acj
Q-score: 0.422
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
Nat Commun (2021) 12 pp. 4909-4909 [ DOI: doi:10.1038/s41467-021-24881-4 Pubmed: 34389707 ]
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- Transfer-messanger rna (tmrna) (117 kDa, RNA from Escherichia coli BL21(DE3))
- Structure of translocated trans-translation complex on e. coli stalled ribosome. (Complex)
- Ribosomal rna and nascent peptide (Complex from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Nascent peptide (236 Da, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- Rna (Complex from Escherichia coli BL21(DE3))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- Smpb (Complex from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
Structure of post-translocated trans-translation complex on E. coli stalled ribosome.
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 7acr
Q-score: 0.38
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
Nat Commun (2021) 12 pp. 4909-4909 [ DOI: doi:10.1038/s41467-021-24881-4 Pubmed: 34389707 ]
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- Transfer-messenger rna (tmrna) (117 kDa, RNA from Escherichia coli)
- Nascent peptide (236 Da, Protein from Escherichia coli K-12)
- Tmrna (Complex from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- Structure of post-translocated trans-translation complex on e. coli stalled ribosome. (Complex)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (17 kDa, Protein from Escherichia coli)
- Ssra-binding protein (Complex from Escherichia coli)
- Ribosome (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
Elongation factor G-ribosome complex captures in the absence of inhibitors.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5ot7
Q-score: 0.344
Mace K, Giudice E, Chat S, Gillet R
Nucleic Acids Res (2018) 46 pp. 3211-3217 [ Pubmed: 29408956 DOI: doi:10.1093/nar/gky081 ]
- Trna phe (Complex from Escherichia coli)
- 23s ribosomal rna (943 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l5 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l28 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Ribosomal protein bl12 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l25 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l16 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l9 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l24 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Elongation factor g (Complex from Thermus thermophilus)
- Ef-g bound to 70s ribosome (Complex)
- 30s ribosomal protein s17 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l29 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l33 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 70s ribosome (Complex from Thermus thermophilus HB8)
- Mrna (4 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l27 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l2 (30 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (Complex from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l13 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l6 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l22 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l4 (23 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Elongation factor g (76 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l15 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Ribosomal protein ul11 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l19 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trna-phe (24 kDa, RNA from Escherichia coli)
- Ribosomal protein ul10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l14 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l30 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l17 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (27 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s ribosomal rna (489 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l23 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l36 (4 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l31 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l32 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l21 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 5s ribosomal rna (38 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l34 (5 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Guanosine-5'-diphosphate (443 Da, Ligand)
tmRNA-SmpB: a journey to the center of the bacterial ribosome
Resolution: 13.0 Å
EM Method: Single-particle
Fitted PDBs: 3iyq
Q-score: 0.074
Weis F, Bron P, Giudice E, Rolland JP, Thomas D, Felden B, Gillet R
EMBO J (2010) 29 pp. 3810-3818 [ DOI: doi:10.1038/emboj.2010.252 Pubmed: 20953161 ]
- Thermus thermophilus 70s ribosome (2 MDa, Sample)
- 70s ribosome (2 MDa, Complex from Thermus thermophilus)
tmRNA-SmpB: a journey to the center of the bacterial ribosome
Resolution: 13.0 Å
EM Method: Single-particle
Fitted PDBs: 3iyr
Q-score: 0.073
Weis F, Bron P, Giudice E, Rolland JP, Thomas D, Felden B, Gillet R
EMBO J (2010) 29 pp. 3810-3818 [ DOI: doi:10.1038/emboj.2010.252 Pubmed: 20953161 ]
- Thermus thermophilus 70s ribosome (2 MDa, Sample)
- 70s ribosome (2 MDa, Complex from Thermus thermophilus)