CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm2 (-12A)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36972428 DOI: 10.1073/pnas.2220874120 ]
Image sets:
- Motion-corrected micrographs
- Unaligned micrographs (raw movies)
- Particle images for map 2 (subtracted particles)
- Particle images for map 1
- Non-dose-weighted motion-corrected micrographs
CryoEM of E. coli RNA polymerase Sigma N bound to full duplex DNA fragment dhsU36 (-12T)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36972428 DOI: 10.1073/pnas.2220874120 ]
Image sets:
- Motion-corrected micrographs (dose-weighted)
- Particle images for map 2 (subtracted particles)
- Unaligned micrographs (raw movies)
- Particle images for map 1
CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm1 (-12T)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36972428 DOI: 10.1073/pnas.2220874120 ]
Image sets:
- Motion-corrected micrographs (dose-weighted)
- Particle images for map 2 (subtracted particles)
- Unaligned micrographs (raw movies)
- Particle images for map 1
EsN-dhsU36mm2 full map (map 1)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
- Esn-dhsu36mm2 (Complex)
- Sigma n / sigma 54 (Protein from Escherichia coli)
- Rna polymerase alpha subunit (Protein from Escherichia coli)
- Rna polymerase omega subunit (Protein from Escherichia coli)
- Rna polymerase beta' subunit (Protein from Escherichia coli)
- Dhsu36mm2 bottom strand (DNA from Aquifex aeolicus)
- Rna polymerase beta subunit (Protein from Escherichia coli)
- Dhsu36mm2 top strand (DNA from Aquifex aeolicus)
EsN-dhsU36mm2 local map (map 2)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
- Dna (Complex from Aquifex aeolicus)
- Esn-dhsu36mm2 (Complex)
- Rna polymerase complex (Complex from Escherichia coli)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8f1j
Q-score: 0.511
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
- Dna (Complex from Aquifex aeolicus)
- Esn-dhsu36mm2 (Complex)
- Rna polymerase complex (Complex from Escherichia coli)
EsN-dhsU36duplex full map (map 1)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
- Rna polymerase alpha subunit (Protein from Escherichia coli)
- Rna polymerase omega subunit (Protein from Escherichia coli)
- Dhsu36duplex bottom strand (DNA from Aquifex aeolicus)
- Rna polymerase beta' subunit (Protein from Escherichia coli)
- Rna polymerase beta subunit (Protein from Escherichia coli)
- Rna polymerase sigma-54 factor (Protein from Escherichia coli)
- Esn-dhsu36duplex (Complex)
- Dhsu36duplex top strand (DNA from Aquifex aeolicus)
EsN-dhsU36duplex local map (map 2)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
EsN-dhsU36duplex composite map
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8f1k
Q-score: 0.505
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
EsN-dhsU36mm1 full map (map 1)
Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA
PNAS (2023) 120 pp. e2220874120-e2220874120 [ DOI: doi:10.1073/pnas.2220874120 Pubmed: 36972428 ]
- Sigma n / sigma 54 (Protein from Escherichia coli)
- Rna polymerase alpha subunit (Protein from Escherichia coli)
- Dhsu36mm1 top strand (DNA from Aquifex aeolicus)
- Rna polymerase omega subunit (Protein from Escherichia coli)
- Rna polymerase beta' subunit (Protein from Escherichia coli)
- Rna polymerase beta subunit (Protein from Escherichia coli)
- Dhsu36mm1 bottom strand (DNA from Aquifex aeolicus)
- Esn-dhsu36mm1 (Complex)