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initial_angle_assignment_type:"MAXIMUM LIKELIHOOD" AND xref_links:"pdb" AND natural_source_ncbi_code:"559292" AND author_orcid:"0000-0003-0830-7341"

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EMD-10713

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Early cytoplasmic yeast pre-40S particle (purified with Tsr1 as bait)

Release date: March 18, 2020
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6y7c
Q-score: 0.235

Shayan R, Rinaldi D, Larburu N, Plassart L, Balor S, Bouyssie D, Lebaron S, Marcoux J, Gleizes PE, Plisson-Chastang C

Molecules (2020) 25 [ DOI: doi:10.3390/molecules25051125 Pubmed: 32138239 ]

Sample:

40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Serine/threonine-protein kinase rio2 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Protein ltv1 (53 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Essential nuclear protein 1 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Early cytoplasmic yeast pre-40s particle (purified using tsr1 as bait) (1 MDa, Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein tsr1 (90 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
20s ribosomal rna (558 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-rrna-processing protein pno1 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-4792

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State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles

Release date: June 26, 2019
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 6rbd, 6rbe
Q-score: 0.16

Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B

Nat Commun (2019) 10 pp. 2754-2754 [ Pubmed: 31227701 DOI: doi:10.1038/s41467-019-10678-z ]

Sample:

Dimethyladenosine transferase (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Serine/threonine-protein kinase rio2 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Essential nuclear protein 1 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
20s ribosomal rna (580 kDa, RNA from Saccharomyces cerevisiae S288C)
40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Ribosome biogenesis protein tsr1 (90 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Pre-rrna-processing protein pno1 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
C1-s20deltaloop state 1 of yeast pre-40s particles purified with tsr1-tap as bait, in mutant conditions (deletion of aa 68-78 of rps20) (1 MDa, Complex from Saccharomyces cerevisiae S288c)
40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-4793

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State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles

Release date: June 26, 2019
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6rbe, 6rbd
Q-score: 0.1465

Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B

Nat Commun (2019) 10 pp. 2754-2754 [ Pubmed: 31227701 DOI: doi:10.1038/s41467-019-10678-z ]

Sample:

Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
C2-s20deltaloop state 2 of yeast pre-40s particles purified with tsr1-tap as bait, in mutant conditions (deletion of aa 68-78 of rps20) (1 MDa, Complex from Saccharomyces cerevisiae S288c)
40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae S288c)
40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-4794

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Yeast pre-40S Rps20 DeltaLoop C1-Headonly

Release date: June 26, 2019
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6rbd, 6rbe
Q-score: 0.0285

Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B

Nat Commun (2019) 10 pp. 2754-2754 [ Pubmed: 31227701 DOI: doi:10.1038/s41467-019-10678-z ]

Sample:

C1-s20deltaloop state 1 of yeast pre-40s particles purified with tsr1-tap as bait, in mutant conditions (deletion of aa 68-78 of rps20) 3d reconstruction of the pre-40s head only (signal subtraction of the body domain) (1 MDa, Complex from Saccharomyces cerevisiae S288c)

EMD-4795

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Yeast pre-40S Rps20 DeltaLoop C2-Headonly

Release date: June 26, 2019
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6rbd, 6rbe
Q-score: 0.032

Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B

Nat Commun (2019) 10 pp. 2754-2754 [ Pubmed: 31227701 DOI: doi:10.1038/s41467-019-10678-z ]

Sample:

C2-s20deltaloop state 2 of yeast pre-40s particles purified with tsr1-tap as bait, in mutant conditions (deletion of aa 68-78 of rps20) (1 MDa, Complex from Saccharomyces cerevisiae S288c)

EMD-4796

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yeast pre-40S particle - focus on Dim1

Release date: June 26, 2019
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 6rbd, 6rbe
Q-score: -0.0005

Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B

Nat Commun (2019) 10 pp. 2754-2754 [ Pubmed: 31227701 DOI: doi:10.1038/s41467-019-10678-z ]

Sample:

C2-s20deltaloop state 2 of yeast pre-40s particles purified with tsr1-tap as bait, in mutant conditions (deletion of aa 68-78 of rps20) (1 MDa, Complex from Saccharomyces cerevisiae S288c)

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EMDB [6]

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