Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? Please take 10 minutes to fill in our annual user survey, and help us make the case for why sustaining open data resources is critical for life sciences research. We appreciate your responses prior to closing of the survey on Monday 17th June.
6-Deoxyerythronolide B synthase (DEBS) hybrid module (M3/1) in complex with antibody fragment 1B2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7e
Q-score: 0.449
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 6-deoxyerythronolide-b synthase erya2, modules 3 and 4,eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (187 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs m3/1te and antibody fragment 1b2 (430 kDa, Complex from Saccharopolyspora erythraea)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m7f
Q-score: 0.403
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7g
Q-score: 0.181
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2: State 1'
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7m7h
Q-score: 0.19
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- Eryai,6-deoxyerythronolide-b synthase erya3, modules 5 and 6 chimera (188 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1te and antibody fragment 1b2 (480 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
6-Deoxyerythronolide B synthase (DEBS) module 1 in complex with antibody fragment 1B2 (TE-free)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7m7i
Q-score: 0.304
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (25 kDa, Protein from Homo sapiens)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- 1b2 (light chain) (26 kDa, Protein from Saccharopolyspora erythraea)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7m7j
Q-score: 0.166
Cogan DP, Zhang K, Li X, Li S, Pintilie GD, Roh SH, Craik CS, Chiu W, Khosla C
Science (2021) 374 pp. 729-734 [ DOI: doi:10.1126/science.abi8358 Pubmed: 34735239 ]
- 1b2 (light chain) (25 kDa, Protein from Homo sapiens)
- Eryai (167 kDa, Protein from Saccharopolyspora erythraea)
- 1b2 (heavy chain) (26 kDa, Protein from Homo sapiens)
- Complex between debs (3)m1(2) and antibody fragment 1b2 (440 kDa, Complex from Saccharopolyspora erythraea)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7fd4
Q-score: 0.4
Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj7835-eabj7835 [ Pubmed: 34652947 DOI: doi:10.1126/sciadv.abj7835 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (Complex)
- A complete three-dimensional structure of the lon protease translocating a protein substrate (conformation 1) (370 kDa, Complex from Meiothermus taiwanensis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Lon protease (Complex from Bos taurus)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7fd5
Q-score: 0.397
Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj7835-eabj7835 [ Pubmed: 34652947 DOI: doi:10.1126/sciadv.abj7835 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- A complete three-dimensional structure of the lon protease translocating a protein substrate (conformation 2) (370 kDa, Complex from Meiothermus taiwanensis)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Lon protease (Complex from Bos taurus)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
Apo L-21 ScaI Tetrahymena ribozyme
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7ez0
Q-score: 0.425
Holo L-16 ScaI Tetrahymena ribozyme
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 7ez2
Q-score: 0.485
Su Z, Zhang K, Kappel K, Li S, Palo MZ, Pintilie GD, Rangan R, Luo B, Wei Y, Das R, Chiu W
Nature (2021) 596 pp. 603-607 [ DOI: doi:10.1038/s41586-021-03803-w Pubmed: 34381213 ]
- Holo l-16 scai tetrahymena ribozyme s1 (2 kDa, RNA from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Holo l-16 scai tetrahymena ribozyme s2 (1 kDa, RNA from Tetrahymena thermophila)
- Holo l-16 scai tetrahymena ribozyme (126 kDa, RNA from Tetrahymena thermophila)
- Holo l-16 scai tetrahymena ribozyme with substrates s1 and s2, and metal ions. (Complex from Tetrahymena thermophila)