The Tet-S2 state of wild-type Tetrahymena group I intron with 30nt 3'/5'-exon
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7xd5
Q-score: 0.504
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- Spermidine (145 Da, Ligand)
- The tet-s2 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (5'-exon) (1 kDa, RNA from Tetrahymena thermophila)
- The tet-s2 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (intron and 3'-exon) (129 kDa, RNA from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7xd6
Q-score: 0.476
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- The tet-s2 state with a pseudoknotted 4-way junction molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (intron and 3'-exon) (140 kDa, RNA from Tetrahymena thermophila)
- Spermidine (145 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- The tet-s2 state with a pseudoknotted 4-way junction molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (5'-exon) (8 kDa, RNA from Tetrahymena thermophila)
The relaxed pre-Tet-S1 state of wild-type Tetrahymena group I intron with 6nt 3'/5'-exon
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 7xd3
Q-score: 0.335
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Magnesium ion (24 Da, Ligand)
- Co-transcriptional folded wild-type tetrahymena group i intron with 6nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- The relaxed pre-tet-s1 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 6nt 3'/5'-exon (137 kDa, RNA from Tetrahymena thermophila)
The intermediate pre-Tet-S1 state of wild-type Tetrahymena group I intron with 6nt 3'/5'-exon
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 7xd4
Q-score: 0.32
The pre-Tet-C state of wild-type Tetrahymena group I intron with 30nt 3'/5'-exon
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 7xd7
Q-score: 0.473
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- The pre-tet-c state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (130 kDa, RNA from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Spermidine (145 Da, Ligand)
Cryo-EM structure of Tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 7ygc
Q-score: 0.454
Li S, Palo MZ, Zhang X, Pintilie G, Zhang K
Nat Commun (2023) 14 pp. 1294-1294 [ Pubmed: 36928031 DOI: doi:10.1038/s41467-023-36724-5 ]
- Rna (5'-r(*cp*cp*cp*up*cp*up*up*ap*ap*cp*c)-3') (3 kDa, RNA from Tetrahymena thermophila)
- Cryo-em structure of tetrahymena ribozyme conformation 4 undergoing the second-step self-splicing (130 kDa, Complex from Tetrahymena)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*up*cp*g)-3') (911 Da, RNA from Tetrahymena thermophila)
- Rna (393-mer) (127 kDa, RNA from Tetrahymena thermophila)
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8hd6
Q-score: 0.319
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- The relaxed pre-tet-s1 state molecule of co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (135 kDa, RNA from Tetrahymena thermophila)
- Co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 5'/3'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Spermidine (145 Da, Ligand)
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8hd7
Q-score: 0.364
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- The intermediate pre-tet-s1 state molecule of co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (135 kDa, RNA from Tetrahymena thermophila)
- Spermidine (145 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Co-transcriptional folded g264a mutated tetrahymena group i intron with 6-nt 3'/5'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8i7n
Q-score: 0.518
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Spermidine (145 Da, Ligand)
- Co-transcriptional folded g264a mutated tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
- The tet-s1 state molecule of co-transcriptional folded g264a mutated tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (134 kDa, RNA from Tetrahymena thermophila)
- (2r,3r,4s,5r)-2-(2-azanylpurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol (267 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Misfolded Tetrahymena ribozyme conformation 1
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7xsk
Q-score: 0.302
Li S, Palo MZ, Pintilie G, Zhang X, Su Z, Kappel K, Chiu W, Zhang K, Das R
PNAS (2022) 119 pp. e2209146119-e2209146119 [ Pubmed: 36067294 DOI: doi:10.1073/pnas.2209146119 ]
- Misfolded tetrahymena ribozyme conformation 1 (120 kDa, Complex from Tetrahymena thermophila)
- Rna (388-mer) (125 kDa, RNA from Tetrahymena thermophila)