Apo Hantaan virus polymerase in monomeric state
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8qe5
Q-score: 0.625
Durieux Trouilleton Q, Housset D, Tarillon P, Arragain B, Malet H
Nat Commun (2024) 15 pp. 2256-2256 [ DOI: doi:10.1038/s41467-024-46601-4 Pubmed: 38480734 ]
- Rna-directed rna polymerase l (249 kDa, Protein from Hantaan virus 76-118)
- Hantaan virus polymerase (250 kDa, Complex from Hantaan virus 76-118)
Resolution: 2.42 Å
EM Method: Single-particle
Fitted PDBs: 8psz
Q-score: 0.647
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Water (18 Da, Ligand)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- [(2~{r},3~{s},4~{r},5~{r})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-~{n}-[oxidanyl(phosphonooxy)phosphoryl]phosphonamidic acid (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
- Transcription-like product (6 kDa, RNA from Tilapia lake virus)
Tilapia Lake Virus polymerase in vRNA initiation state (transcriptase conformation)
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8psn
Q-score: 0.632
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Water (18 Da, Ligand)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
- Dna (5'-d(*(ctp))-3') (483 Da, DNA from synthetic construct)
Tilapia Lake Virus polymerase in vRNA initiation state (core only)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8pso
Q-score: 0.679
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Water (18 Da, Ligand)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
- Dna (5'-d(*(ctp))-3') (483 Da, DNA from synthetic construct)
Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core only)
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8psq
Q-score: 0.648
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' crna end - crna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Water (18 Da, Ligand)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
Tilapia Lake Virus polymerase in cRNA pre-initiation state mode B (core-endo only)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8pss
Q-score: 0.586
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' crna end - crna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode A (core only)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8psu
Q-score: 0.572
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
Tilapia Lake Virus polymerase in vRNA elongation state (transcriptase conformation)
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8psx
Q-score: 0.595
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- [(2~{r},3~{s},4~{r},5~{r})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-~{n}-[oxidanyl(phosphonooxy)phosphoryl]phosphonamidic acid (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
- Transcription-like product (6 kDa, RNA from Tilapia lake virus)
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation)
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 8pt2
Q-score: 0.635
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Water (18 Da, Ligand)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
Tilapia Lake Virus polymerase in vRNA initiation state (replicase conformation)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8pt6
Q-score: 0.513
Arragain B, Pelosse M, Thompson A, Cusack S
Nat Commun (2023) 14 pp. 8145-8145 [ Pubmed: 38066000 DOI: doi:10.1038/s41467-023-44044-x ]
- 5' vrna end - vrna loop (40-mer) (12 kDa, RNA from Tilapia lake virus)
- Putative pb1 (57 kDa, Protein from Tilapia lake virus)
- Rna-dependent rna polymerase (53 kDa, Protein from Tilapia lake virus)
- Polymerase acidic protein (pa-like) (47 kDa, Protein from Tilapia lake virus)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Tilapia lake virus polymerase (Complex from Tilapia lake virus)
- Dna (5'-d(*(ctp))-3') (483 Da, DNA from synthetic construct)