Resolution: 1.9 Å
EM Method: Electron Crystallography
Fitted PDBs: 5knz
Q-score: 0.801
Krotee P, Rodriguez JA, Sawaya MR, Cascio D, Reyes FE, Shi D, Hattne J, Nannenga BL, Oskarsson ME, Philipp S, Griner S, Jiang L, Glabe CG, Westermark GT, Gonen T, Eisenberg DS
eLife (2017) 6 [ DOI: doi:10.7554/eLife.19273 Pubmed: 28045370 ]
- Hiapp(residues 19-29)s20g (1 kDa, Protein from Homo sapiens)
- Amyloid fiber (4 MDa, Complex)
- Water (18 Da, Ligand)
Human Islet Amyloid Polypeptide Segment 15-FLVHSSNNFGA-25 Determined by MicroED
Resolution: 1.4 Å
EM Method: Electron Crystallography
Fitted PDBs: 5ko0
Q-score: 0.377
Krotee P, Rodriguez JA, Sawaya MR, Cascio D, Reyes FE, Shi D, Hattne J, Nannenga BL, Oskarsson ME, Philipp S, Griner S, Jiang L, Glabe CG, Westermark GT, Gonen T, Eisenberg DS
eLife (2017) 6 [ DOI: doi:10.7554/eLife.19273 Pubmed: 28045370 ]
- Water (18 Da, Ligand)
- Hiapp(15-25)wt (1 kDa, Protein from Homo sapiens)
- Thiocyanate ion (58 Da, Ligand)
- Amyloid fiber (4 MDa, Complex)
Sawicka M, Wanrooij PH, Darbari VC, Tannous E, Hailemariam S, Bose D, Makarova AV, Burgers PM, Zhang X
J Biol Chem (2016) 291 pp. 13436-13447 [ Pubmed: 27129217 DOI: doi:10.1074/jbc.M115.708263 ]
Electron cryo-microscopy of an dsRNA-empty totivirus-like Omono River virus (OmRV)
Okamoto K, Miyazaki N, Larsson DSD, Kobayashi D, Svenda M, Muehlig K, Maia FRNC, Gunn L-H, Isawa H, Kobayashi M, Sawabe K, Murata K, Hajdu J
Sci Rep (2016) 6 [ Pubmed: 27616740 DOI: doi:10.1038/srep33170 ]
Electron cryo-microscopy of an dsRNA-full Omono River virus (OmRV)
Okamoto K, Miyazaki N, Larsson DSD, Kobayashi D, Svenda M, Muehlig K, Maia FRNC, Gunn L-H, Isawa H, Kobayashi M, Sawabe K, Murata K, Hajdu J
Sci Rep (2016) 6 pp. 33170- [ Pubmed: 27616740 DOI: doi:10.1038/srep33170 ]
Structure of NNQQNY from yeast prion Sup35 with zinc acetate determined by MicroED
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
PNAS (2016) 113 pp. 11232-11236 [ Pubmed: 27647903 DOI: doi:10.1073/pnas.1606287113 ]
- Prion fibril composed of a 6-residue segment of sup35 and zinc (3 MDa, Complex from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- Eukaryotic peptide chain release factor gtp-binding subunit (779 Da, Protein from Saccharomyces cerevisiae)
- Acetic acid (60 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Structure of NNQQNY from yeast prion Sup35 with cadmium acetate determined by MicroED
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
PNAS (2016) 113 pp. 11232-11236 [ Pubmed: 27647903 DOI: doi:10.1073/pnas.1606287113 ]
- Cadmium ion (112 Da, Ligand)
- Water (18 Da, Ligand)
- Eukaryotic peptide chain release factor gtp-binding subunit (779 Da, Protein from Saccharomyces cerevisiae)
- Acetate ion (59 Da, Ligand)
- Prion fibril composed of a 6-residue segment of sup35 and cadmium (3 MDa, Complex from Saccharomyces cerevisiae)
Structure of GNNQQNY from yeast prion Sup35 in space group P21 determined by MicroED
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
PNAS (2016) 113 pp. 11232-11236 [ Pubmed: 27647903 DOI: doi:10.1073/pnas.1606287113 ]
Structure of GNNQQNY from yeast prion Sup35 in space group P212121 determined by MicroED
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
PNAS (2016) 113 pp. 11232-11236 [ Pubmed: 27647903 DOI: doi:10.1073/pnas.1606287113 ]
Boskovic J, Martinez-Gago J, Mendez-Pertuz M, Buscato A, Martinez-Torrecuadrada JL, Blasco MA
J Biol Chem (2016) 291 pp. 21829-21835 [ Pubmed: 27563064 DOI: doi:10.1074/jbc.M116.744896 ]
- Trf1 dimer (100 kDa, Complex from Mus musculus)