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The MicroED structure of proteinase K crystallized by suspended drop crystallization
Resolution: 2.1 Å
EM Method: Electron Crystallography
Fitted PDBs: 8sdk
Q-score: 0.739
Gillman C, Nicolas WJ, Martynowycz MW, Gonen T
IUCrJ (2023) 10 pp. 430-436 [ DOI: doi:10.1107/S2052252523004141 Pubmed: 37223996 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Sulfate ion (96 Da, Ligand)
- Water (18 Da, Ligand)
- Proteinase k from tritirachium album (28 kDa, Cellular component from Parengyodontium album)
MicroED structure of Proteinase K from lamellae milled from multiple plasma sources
Resolution: 1.39 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyo
Q-score: 0.911
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ DOI: doi:10.1038/s41467-023-36733-4 Pubmed: 36841804 ]
- Calcium ion (40 Da, Ligand)
- Nitrate ion (62 Da, Ligand)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
MicroED structure of Proteinase K from xenon milled lamellae
Resolution: 1.45 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyp
Q-score: 0.898
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ DOI: doi:10.1038/s41467-023-36733-4 Pubmed: 36841804 ]
- Calcium ion (40 Da, Ligand)
- Nitrate ion (62 Da, Ligand)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
MicroED structure of Proteinase K from argon milled lamellae
Resolution: 1.4 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyq
Q-score: 0.905
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ DOI: doi:10.1038/s41467-023-36733-4 Pubmed: 36841804 ]
- Calcium ion (40 Da, Ligand)
- Nitrate ion (62 Da, Ligand)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
MicroED structure of Proteinase K from oxygen milled lamellae
Resolution: 1.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fyr
Q-score: 0.894
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ DOI: doi:10.1038/s41467-023-36733-4 Pubmed: 36841804 ]
- Calcium ion (40 Da, Ligand)
- Nitrate ion (62 Da, Ligand)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
MicroED structure of Proteinase K from nitrogen milled lamellae
Resolution: 1.8 Å
EM Method: Electron Crystallography
Fitted PDBs: 8fys
Q-score: 0.833
Martynowycz MW, Shiriaeva A, Clabbers MTB, Nicolas WJ, Weaver SJ, Hattne J, Gonen T
Nat Commun (2023) 14 pp. 1086-1086 [ DOI: doi:10.1038/s41467-023-36733-4 Pubmed: 36841804 ]
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
MicroED structure of proteinase K recorded on K2
Clabbers MTB, Martynowycz MW, Hattne J, Nannenga BL, Gonen T
J Struct Biol (2022) 214 pp. 107886-107886 [ DOI: doi:10.1016/j.jsb.2022.107886 Pubmed: 36044956 ]
- Calcium ion (40 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
MicroED structure of proteinase K recorded on K3
Clabbers MTB, Martynowycz MW, Hattne J, Nannenga BL, Gonen T
J Struct Biol (2022) 214 pp. 107886-107886 [ DOI: doi:10.1016/j.jsb.2022.107886 Pubmed: 36044956 ]
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV
Martynowycz MW, Clabbers MTB, Unge J, Hattne J, Gonen T
PNAS (2021) 118 [ Pubmed: 34873060 DOI: doi:10.1073/pnas.2108884118 ]
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)
MicroED structure of proteinase K from a 200 nm thick lamella measured at 120 kV
Martynowycz MW, Clabbers MTB, Unge J, Hattne J, Gonen T
PNAS (2021) 118 [ Pubmed: 34873060 DOI: doi:10.1073/pnas.2108884118 ]
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Protein from Parengyodontium album)
- Proteinase k (28 kDa, Complex from Parengyodontium album)