Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pfo
Q-score: 0.391
Jones ML, Baris Y, Taylor MRG, Yeeles JTP
EMBO J (2021) 40 pp. e108819-e108819 [ Pubmed: 34694004 DOI: doi:10.15252/embj.2021108819 ]
- Dna polymerase epsilon subunit 2 (59 kDa, Protein from Homo sapiens)
- Dna replication complex gins protein psf1 (23 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication complex gins protein psf2 (21 kDa, Protein from Homo sapiens)
- Wd repeat and hmg-box dna-binding protein 1 (130 kDa, Protein from Homo sapiens)
- Leading strand dna (26 kDa, DNA from Homo sapiens)
- Dna replication complex gins protein psf3 (24 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cell division control protein 45 homolog (66 kDa, Protein from Homo sapiens)
- Core human replisome (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna replication complex gins protein sld5 (30 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Sulfate ion (96 Da, Ligand)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Protein timeless homolog (138 kDa, Protein from Homo sapiens)
- Lagging strand dna (16 kDa, DNA from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna polymerase epsilon catalytic subunit a (261 kDa, Protein from Homo sapiens)
- Claspin (155 kDa, Protein from Homo sapiens)
- Timeless-interacting protein (34 kDa, Protein from Homo sapiens)
H. sapiens replisome-CUL2/LRR1 complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7plo
Q-score: 0.415
Jenkyn-Bedford M, Jones ML, Baris Y, Labib KPM, Cannone G, Yeeles JTP, Deegan TD
Nature (2021) 600 pp. 743-747 [ DOI: doi:10.1038/s41586-021-04145-3 Pubmed: 34700328 ]
- Lagging strand dna (12 kDa, DNA from Homo sapiens)
- Dna polymerase epsilon subunit 2 (59 kDa, Protein from Homo sapiens)
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Dna replication complex gins protein psf1 (23 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Leucine-rich repeat protein 1 (46 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- Dna replication complex gins protein psf2 (21 kDa, Protein from Homo sapiens)
- Wd repeat and hmg-box dna-binding protein 1 (130 kDa, Protein from Homo sapiens)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Leading strand dna (26 kDa, DNA from Homo sapiens)
- Dna replication complex gins protein psf3 (24 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cell division control protein 45 homolog (66 kDa, Protein from Homo sapiens)
- Human replisome engaged with cul2-lrr1 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna replication complex gins protein sld5 (30 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Sulfate ion (96 Da, Ligand)
- Protein timeless homolog (138 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna polymerase epsilon catalytic subunit a (261 kDa, Protein from Homo sapiens)
- Claspin (155 kDa, Protein from Homo sapiens)
- Timeless-interacting protein (34 kDa, Protein from Homo sapiens)
S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pmk
Q-score: 0.434
Jenkyn-Bedford M, Jones ML, Baris Y, Labib KPM, Cannone G, Yeeles JTP, Deegan TD
Nature (2021) 600 pp. 743-747 [ DOI: doi:10.1038/s41586-021-04145-3 Pubmed: 34700328 ]
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45,cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome segregation in meiosis protein 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Dna helicase (86 kDa, Protein from Saccharomyces cerevisiae)
- Lagging strand template dna (35 kDa, DNA from Synthetic construct)
- Replisome (Complex from Saccharomyces cerevisiae)
- E3 ubiquitin ligase complex scf subunit (22 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (22 kDa, Protein from Saccharomyces cerevisiae)
- Dna (Complex from DNA molecule)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Protein dia2 (85 kDa, Protein from Saccharomyces cerevisiae)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Budding yeast replisome on double-stranded dna engaged with scf(dia2) (conformation i) (Complex)
- Magnesium ion (24 Da, Ligand)
- Leading strand template dna (37 kDa, DNA from Synthetic construct)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Topoisomerase 1-associated factor 1 (141 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (108 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pmn
Q-score: 0.405
Jenkyn-Bedford M, Jones ML, Baris Y, Labib KPM, Cannone G, Yeeles JTP, Deegan TD
Nature (2021) 600 pp. 743-747 [ DOI: doi:10.1038/s41586-021-04145-3 Pubmed: 34700328 ]
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Budding yeast replisome on double-stranded dna engaged with scf(dia2) (conformation ii) (Complex)
- Leading strand template dna (37 kDa, DNA from Synthetic construct)
- Cell division control protein 45,cell division control protein 45 (75 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome segregation in meiosis protein 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Replisome (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (22 kDa, Protein from Saccharomyces cerevisiae)
- Lagging strand template dna (35 kDa, DNA from Saccharomyces cerevisiae)
- Dna (Complex from DNA molecule)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Protein dia2 (85 kDa, Protein from Saccharomyces cerevisiae)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Topoisomerase 1-associated factor 1 (141 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha-binding protein (108 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)