SARS-CoV-2 RdRP catalytic complex with T33-1 RNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7dte
Q-score: 0.528
Wu J, Wang H, Liu Q, Li R, Gao Y, Fang X, Zhong Y, Wang M, Wang Q, Rao Z, Gong P
Cell Rep (2021) 37 pp. 109882-109882 [ Pubmed: 34653416 DOI: doi:10.1016/j.celrep.2021.109882 ]
- Sars-cov-2 rna-dependent rna polymerase (Complex)
- Rna (57-mer) (18 kDa, RNA from Foot-and-mouth disease virus)
- Rna (Complex)
- Rna-directed rna polymerase (108 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (33-mer) (10 kDa, RNA from Foot-and-mouth disease virus)
- Zinc ion (65 Da, Ligand)
- Sars-cov-2 rna-dependent rna polymerase catalytic complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
COVID-19 RNA-dependent RNA polymerase post-translocated catalytic complex
Resolution: 3.26 Å
EM Method: Single-particle
Fitted PDBs: 7bzf
Q-score: 0.47
Wang Q, Wu J, Wang H, Gao Y, Liu Q, Mu A, Ji W, Yan L, Zhu Y, Zhu C, Fang X, Yang X, Huang Y, Gao H, Liu F, Ge J, Sun Q, Yang X, Xu W, Liu Z, Yang H, Lou Z, Jiang B, Guddat LW, Gong P, Rao Z
Cell (2020) 182 pp. 417-428.e13 [ DOI: doi:10.1016/j.cell.2020.05.034 Pubmed: 32526208 ]
- Structure of covid-19 rna-dependent rna polymerase in complex with rna (155 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(*up*gp*up*up*cp*gp*ap*cp*gp*ap*cp*ap*cp*a)-3') (4 kDa, RNA from Foot-and-mouth disease virus)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (31-mer) (10 kDa, RNA from Foot-and-mouth disease virus)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase (108 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
COVID-19 RNA-dependent RNA polymerase pre-translocated catalytic complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7c2k
Q-score: 0.581
Wang Q, Wu J, Wang H, Gao Y, Liu Q, Mu A, Ji W, Yan L, Zhu Y, Zhu C, Fang X, Yang X, Huang Y, Gao H, Liu F, Ge J, Sun Q, Yang X, Xu W, Liu Z, Yang H, Lou Z, Jiang B, Guddat LW, Gong P, Rao Z
Cell (2020) 182 pp. 417-428.e13 [ DOI: doi:10.1016/j.cell.2020.05.034 Pubmed: 32526208 ]
- Rna-directed rna polymerase (108 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Covid-19 rna-dependent rna polymerase pre-translocated catalytic complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (29-mer) (9 kDa, RNA from Foot-and-mouth disease virus)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(*up*gp*up*up*cp*gp*ap*cp*gp*ap*cp*ap*cp*ap*gp*g*(f86)p*g)-3') (5 kDa, RNA from Foot-and-mouth disease virus)
SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors in reduced conditions
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 7btf
Q-score: 0.569
Gao Y, Yan L, Huang Y, Liu F, Zhao Y, Cao L, Wang T, Sun Q, Ming Z, Zhang L, Ge J, Zheng L, Zhang Y, Wang H, Zhu Y, Zhu C, Hu T, Hua T, Zhang B, Yang X, Li J, Yang H, Liu Z, Xu W, Guddat LW, Wang Q, Lou Z, Rao Z
Science (2020) 368 pp. 779-782 [ Pubmed: 32277040 DOI: doi:10.1126/science.abb7498 ]
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Structure of sars-cov-2 rna-dependent rna polymerase in complex with cofactors in reduced condition (155 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase (108 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6m71
Q-score: 0.519
Gao Y, Yan L, Huang Y, Liu F, Zhao Y, Cao L, Wang T, Sun Q, Ming Z, Zhang L, Ge J, Zheng L, Zhang Y, Wang H, Zhu Y, Zhu C, Hu T, Hua T, Zhang B, Yang X, Li J, Yang H, Liu Z, Xu W, Guddat LW, Wang Q, Lou Z, Rao Z
Science (2020) 368 pp. 779-782 [ Pubmed: 32277040 DOI: doi:10.1126/science.abb7498 ]
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 rna-dependent rna polymerase in complex with cofactors (155 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (108 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7w
Q-score: 0.324
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Dna (25-mer) (7 kDa, DNA from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (77-mer) (23 kDa, DNA from Enterobacteria phage T7)
- Trxa (11 kDa, Protein from Escherichia coli)
- D5ae7a mutant gp5 dna polymerase complexed with trx, fork dna, and incoming dttp (from gp4-gp5 leading-strand complexes) (Complex from Enterobacteria phage T7)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6n9u
Q-score: 0.477
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Gp5 dna polymerase and two gp4 primase subunits complexed with trx, rna/dna hybrid, and incoming dttp (from gp4-gp5 lagging-strand complex lags1) (Complex from Enterobacteria phage T7)
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Dna (44-mer) (13 kDa, DNA from Enterobacteria phage T7)
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(*ap*cp*cp*ap*g)-d(p*(doc))-3') (1 kDa, RNA from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n9v
Q-score: 0.408
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Gp5 dna polymerase and two gp4 primase subunits complexed with trx, rna/dna hybrid, and incoming dttp (from gp4-gp5 lagging-strand complex lags1) (Complex from Enterobacteria phage T7)
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6n9w
Q-score: 0.334
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)
- Gp5 dna polymerase binding to a and b subunits of gp4 primase-helicase hexamer complexed with trx, rna/dna hybrid, and incoming dttp (lags2) (Complex from Enterobacteria phage T7)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Thymidine-5'-triphosphate (482 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6n9x
Q-score: 0.371
Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science (2019) 363 [ Pubmed: 30679383 DOI: doi:10.1126/science.aav7003 ]
- Dna primase/helicase (62 kDa, Protein from Enterobacteria phage T7)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Primer (1 kDa, RNA from Enterobacteria phage T7)
- Template (13 kDa, DNA from Enterobacteria phage T7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Gp5 dna polymerase binding to b and c subunits of gp4 primase-helicase hexamer complexed with trx, rna/dna hybrid, and incoming dttp (lags3) (Complex from Enterobacteria phage T7)
- Dna-directed dna polymerase (79 kDa, Protein from Enterobacteria phage T7)