Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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dna/ polymerase AND author_orcid:"0000-0002-2311-003x" AND author_orcid:"0000-0003-0628-211x" AND xref_links:"go"

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EMD-22160

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Structure of SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC

Release date: July 29, 2020
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6xez
Q-score: 0.41

Chen J, Malone B, Llewellyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, Eng ET, Vatandaslar H, Chait BT, Kapoor TM, Darst SA, Campbell EA

Cell (2020) 182 pp. 1560-1573.e13 [ Pubmed: 32783916 DOI: doi:10.1016/j.cell.2020.07.033 ]

Sample:

Chapso (631 Da, Ligand)
Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Aluminum fluoride (83 Da, Ligand)
Template rna (17 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Sars-cov-2 replication/transcription complex bound to nsp13 helicase - nsp13(2)-rtc (Complex from Severe acute respiratory syndrome coronavirus 2)
Adenosine-5'-diphosphate (427 Da, Ligand)
Magnesium ion (24 Da, Ligand)
Zinc ion (65 Da, Ligand)
Product rna (11 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)

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