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Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
To Be Published
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Magnesium ion (24 Da, Ligand)
- Phalloidin. (Complex from Amanita phalloides)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin filament (Complex from Homo sapiens)
Cryo-EM reconstruction of the formin Cdc12 bound to the barbed end of F-actin (without phalloidin)
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Human cytoplasmic beta-actin (Protein from Homo sapiens)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of f-actin. (Complex from Homo sapiens)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- S. pombe cdc12 (Protein from Schizosaccharomyces pombe)
- Actin filament (Complex)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Actin filament (Complex from Homo sapiens)
Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
- Actin filament (Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Structure of the F-actin barbed end bound by formin mDia1
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8ru2
Q-score: 0.362
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,protein diaphanous homolog 1 (86 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Mdia1-bound f-actin barbed end. (Complex)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Actin filament (Complex from Homo sapiens)
- Mouse mdia1 (fh1fh2c domain) (Complex from Mus musculus)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Inf2 dimer (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K1179W mutant
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8cpz
Q-score: 0.526
Nganga PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Assafa TE, Kuehnemuth R, Dong M, Seidel C, Bordignon E, Raunser S
To Be Published
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, K567W K2008W mutant
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8cq0
Q-score: 0.466
Nganga PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Assafa TE, Kuehnemuth R, Dong M, Seidel C, Bordignon E, Raunser S
To Be Published
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Photorhabdus luminescens TcdA1 prepore-to-pore intermediate, C16S, C20S, C870S, T1279C mutant
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8cq2
Q-score: 0.448
Nganga PN, Folz J, Kucher S, Roderer D, Xu Y, Sitsel O, Belyy A, Prumbaum D, Assafa TE, Kuehnemuth R, Dong M, Seidel C, Bordignon E, Raunser S
To Be Published
- Tcda1 (1 MDa, Complex from Photorhabdus luminescens)
- Tcda1 (285 kDa, Protein from Photorhabdus luminescens)
Structure of the human inner kinetochore CCAN complex
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7qoo
Q-score: 0.202
Pesenti ME, Raisch T, Conti D, Walstein K, Hoffmann I, Vogt D, Prumbaum D, Vetter IR, Raunser S, Musacchio A
Mol Cell (2022) 82 pp. 2113- [ DOI: doi:10.1016/j.molcel.2022.04.027 Pubmed: 35525244 ]
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Human inner kinetochore ccan complex (451 kDa, Complex from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Centromere protein t (60 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Unknown protein (1 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Centromere protein w (10 kDa, Protein from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)