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Cryo-EM structure of consensus elemental paused elongation complex
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36795753 DOI: 10.1073/pnas.2215945120 ]
Image sets:
Cryo-EM structure of his-elemental paused elongation complex
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36795753 DOI: 10.1073/pnas.2215945120 ]
Image sets:
Cryo-EM structure of pre-consensus elemental paused elongation complex
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
Proc Natl Acad Sci U S A (2023) 120 [ Pubmed: 36795753 DOI: 10.1073/pnas.2215945120 ]
Image sets:
Cryo-EM structure of pre-consensus elemental paused elongation complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8eg7
Q-score: 0.504
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Pre-consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (5 kDa, RNA from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of consensus elemental paused elongation complex with a folded TL
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8eg8
Q-score: 0.507
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Rna (5 kDa, RNA from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Consensus elemental paused elongation complex with a folded tl (450 kDa, Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of consensus elemental paused elongation complex with an unfolded TL
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8egb
Q-score: 0.434
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Rna (5 kDa, RNA from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Consensus elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8eh8
Q-score: 0.496
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8eh9
Q-score: 0.429
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8eha
Q-score: 0.461
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8ehf
Q-score: 0.494
Kang JY, Mishanina TV, Bao Y, Chen J, Llewellyn E, Liu J, Darst SA, Landick R
PNAS (2023) 120 pp. e2215945120-e2215945120 [ DOI: doi:10.1073/pnas.2215945120 Pubmed: 36795753 ]
- Dna-directed rna polymerase subunit alpha (26 kDa, Protein from Escherichia coli)
- Template dna (9 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- (3r,5s,7r,8r,9s,10s,12s,13r,14s,17r)-10,13-dimethyl-17-[(2r)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triol (378 Da, Ligand)
- His-elemental paused elongation complex (450 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Non-template dna (9 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)