Cryo-electron tomography of a Golgi intracisternal protein array
Engel BD, Schaffer M, Albert S, Asano S, Plitzko JM, Baumeister W
PNAS (2015) 112 pp. 11264-11269 [ DOI: doi:10.1073/pnas.1515337112 Pubmed: 26311849 ]
Structure of the 26S proteasome-Ubp6 complex
Resolution: 9.5 Å
EM Method: Single-particle
Fitted PDBs: 5a5b
Q-score: 0.071
Aufderheide A, Beck F, Stengel F, Hartwig M, Schweitzer A, Pfeifer G, Goldberg AL, Sakata E, Baumeister W, Foerster F
PNAS (2015) 112 pp. 8626-8631 [ Pubmed: 26130806 DOI: doi:10.1073/pnas.1510449112 ]
- 26s proteasome from saccharomyces cerevisiae in the presence of saccharomyces cerevisiae ubp6 and ubiquitin aldehyde (2 MDa, Sample)
- 26s proteasome (2 MDa, Protein from Saccharomyces cerevisiae)
- Ubp6 (60 kDa, Protein from Saccharomyces cerevisiae)
- Ubiqutin aldehyde (Protein from Homo sapiens)
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Resolution: 6.6 Å
EM Method: Single-particle
Fitted PDBs: 4v1m
Q-score: 0.151
Plaschka C, Lariviere L, Wenzeck L, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F, Villa E, Cramer P
Nature (2015) 518 pp. 376-380 [ DOI: doi:10.1038/nature14229 Pubmed: 25652824 ]
- Template dna (DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae)
- Nontemplate dna (DNA from synthetic construct)
- Rna (RNA from synthetic construct)
- Pol ii-dna/rna (570 kDa, Sample)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 3 (17 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb1 (192 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (18 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb2 (139 kDa, Protein from Saccharomyces cerevisiae)
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 4v1n
Q-score: 0.129
Plaschka C, Lariviere L, Wenzeck L, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F, Villa E, Cramer P
Nature (2015) 518 pp. 376-380 [ Pubmed: 25652824 DOI: doi:10.1038/nature14229 ]
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Citc (750 kDa, Sample)
- Dna-directed rna polymerase ii subunit rpb1 (192 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (8 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces mikatae)
- Dna-directed rna polymerase ii subunit rpb2 (139 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit beta (47 kDa, Protein from Saccharomyces cerevisiae)
- Rna (RNA from synthetic construct)
- Nontemplate dna (DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 3 (17 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
- Tata-box-binding protein (20 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (18 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae)
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Resolution: 9.7 Å
EM Method: Single-particle
Fitted PDBs: 4v1o
Q-score: 0.116
Plaschka C, Lariviere L, Wenzeck L, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F, Villa E, Cramer P
Nature (2015) 518 pp. 376-380 [ Pubmed: 25652824 DOI: doi:10.1038/nature14229 ]
- Mediator of rna polymerase ii transcription subunit 7 (26 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 19 (25 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb1 (192 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (8 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces mikatae)
- Mediator of rna polymerase ii transcription subunit 10 (18 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 31 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb2 (139 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit beta (47 kDa, Protein from Saccharomyces cerevisiae)
- Rna (RNA from synthetic construct)
- Nontemplate dna (DNA from synthetic construct)
- Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae)
- Citc-cmed (1 MDa, Sample)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 3 (17 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 6 (33 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 22 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
- Tata-box-binding protein (20 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (18 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 17 (79 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 4 (32 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 14 (85 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 20 (23 kDa, Protein from Saccharomyces cerevisiae)
Asano S, Fukuda Y, Beck F, Aufderheide A, Foerster F, Danev R, Baumeister W
Science (2015) 347 pp. 439-442 [ DOI: doi:10.1126/science.1261197 Pubmed: 25613890 ]
Asano S, Fukuda Y, Beck F, Aufderheide A, Foerster F, Danev R, Baumeister W
Science (2015) 347 pp. 439-442 [ DOI: doi:10.1126/science.1261197 Pubmed: 25613890 ]