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EMD-8826

Single-particle
3.6 Å
EMD-8826
3D View Gallery
Deposition: 11/07/2017
Map released: 02/05/2018
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8826
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5wf0 summary report (pdf.gz) Map and 5wf0 FULL report (pdf.gz)
EMICSS annotations (xml)

70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex I)

EMD-8826

Single-particle
3.6 Å
EMD-8826
3D View Gallery
Deposition: 11/07/2017
Map released: 02/05/2018
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, Escherichia coli O139:H28 (strain E24377A / ETEC)
Sample: 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex 1)
Fitted models: 5wf0

Deposition Authors: Fislage M , Frank J
Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
Fislage M , Zhang J, Brown ZP , Mandava CS , Sanyal S, Ehrenberg M, Frank J
(2018) Nucleic Acids Res , 46 , 5861 - 5874
PUBMED: 29733411
DOI: doi:10.1093/nar/gky346
ISSN: 1362-4962
ASTM: NARHAD