EMD-65732

Single-particle
3.91 Å
EMD-65732
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 31/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-65732
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9w7t summary report (pdf.gz) Map and 9w7t FULL report (pdf.gz)
EMICSS annotations (xml)

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B

EMD-65732

Single-particle
3.91 Å
EMD-65732
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 31/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes, Streptococcus pyogenes serotype M1
Sample: SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B
Fitted models: 9w7t

Deposition Authors: Zheng R, Ma LJ

5' extended gRNA increases the cleavage activity of SuperFi-Cas9
Zheng R, Ma LJ
To Be Published
Grant Support: