EMD-65250

Helical reconstruction
3.11 Å
EMD-65250
3D View Gallery
Deposition: 03/07/2025
Map released: 24/12/2025
Last modified: 08/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-65250

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9vpe summary report (pdf.gz) Map-9vpe FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

CryoEM structure of cyclised H-pilus (D69G)

EMD-65250

Helical reconstruction
3.11 Å
EMD-65250
3D View Gallery
Deposition: 03/07/2025
Map released: 24/12/2025
Last modified: 08/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Salmonella enterica subsp. enterica serovar Typhi
Sample: H-Pilus (D69G)
Fitted models: 9vpe

Deposition Authors: Ishimoto N , Beis K
H pilin cyclisation and pilus biogenesis are promiscuous but electrostatic perturbations impair conjugation efficiency.
He S , Ishimoto N , Wong JLC , David S , Sanchez-Garrido J , Bogdanov M , Beis K , Frankel G
(2026) Nat Commun , 17
PUBMED: 41708604
DOI: doi:10.1038/s41467-026-69599-3
ISSN: 2041-1723
Grant Support: