EMD-63066

Single-particle
2.86 Å
EMD-63066
3D View Gallery
Deposition: 10/01/2025
Map released: 20/08/2025
Last modified: 20/08/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-63066

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9lgi summary report (pdf.gz) Map-9lgi FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of a type II-D CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA

EMD-63066

Single-particle
2.86 Å
EMD-63066
3D View Gallery
Deposition: 10/01/2025
Map released: 20/08/2025
Last modified: 20/08/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Nitrospirae bacterium RBG_13_39_12, synthetic construct
Sample: Ternary complex of NsCas9-sgRNA-dsDNA
Fitted models: 9lgi

Deposition Authors: Wang K , Wang Y
Structural insights into Type II-D Cas9 and its robust cleavage activity.
Wang K , Wang J , Yang X , Sun W , Sheng G, Wang Y
(2025) Nat Commun , 16 , 7396 - 7396
PUBMED: 40790018
DOI: doi:10.1038/s41467-025-62128-8
ISSN: 2041-1723