EMD-54254

Single-particle
3.6 Å
EMD-54254
3D View Gallery
Deposition: 03/07/2025
Map released: 29/10/2025
Last modified: 12/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-54254
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9rtv summary report (pdf.gz) Map and 9rtv FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of 70S ribosome-EF-G(P610L)-GDP-Pi-apramycin complex with tRNAs in hybrid state 2 (H2- EF-G(P610L)-GDP-Pi)

EMD-54254

Single-particle
3.6 Å
EMD-54254
3D View Gallery
Deposition: 03/07/2025
Map released: 29/10/2025
Last modified: 12/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli K-12
Sample: E. coli ribosome complex 70S-Apr-EF-G(P610L)-GDP-Pi-fMet-Phe-tRNAPhe-tRNA-fMet-mRNA
Fitted models: 9rtv

Deposition Authors: Ghosh Dastidar N, Freyer N, Petrychenko V, Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi A, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I
Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides.
Ghosh Dastidar N, Freyer NS, Petrychenko V, de A P Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi AZ, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I
(2025) Nat Commun , 16 , 9568 - 9568
PUBMED: 41162373
DOI: doi:10.1038/s41467-025-65298-7
ISSN: 2041-1723