EMD-53854

Single-particle
3.1 Å
EMD-53854
3D View Gallery
Deposition: 19/05/2025
Map released: 30/07/2025
Last modified: 03/09/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-53854
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9r96 summary report (pdf.gz) Map and 9r96 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA (pppGpGpA) and GTP poised for catalysis (slipped pre-IC4)

EMD-53854

Single-particle
3.1 Å
EMD-53854
3D View Gallery
Deposition: 19/05/2025
Map released: 30/07/2025
Last modified: 03/09/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Homo sapiens, synthetic construct
Sample: human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA (pppGpGpA) and GTP poised for catalysis (slipped pre-IC4)
Fitted models: 9r96

Deposition Authors: Goovaerts Q , Shen J, Ajjugal Y, De Wijngaert B, Patel SS , Das K
Human mitochondrial RNA polymerase structures reveal transcription start site and slippage mechanism.
Shen J, Goovaerts Q , Ajjugal Y, De Wijngaert B, Das K , Patel SS
(2025) Mol Cell , 85 , 3137 - 3150.e7
PUBMED: 40712586
DOI: doi:10.1016/j.molcel.2025.07.002
ISSN: 1097-2765
ASTM: MOCEFL