EMD-52346

Single-particle
3.51 Å
EMD-52346
3D View Gallery
Deposition: 16/12/2024
Map released: 26/11/2025
Last modified: 26/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-52346
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9hqp summary report (pdf.gz) Map and 9hqp FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of mouse TMEM16F-YFP purified and plunged using MISO (microfluidic isolation)

EMD-52346

Single-particle
3.51 Å
EMD-52346
3D View Gallery
Deposition: 16/12/2024
Map released: 26/11/2025
Last modified: 26/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Mus musculus, Aequorea victoria
Sample: Scramblase 16F with c-terminal venus GFP
Fitted models: 9hqp

Deposition Authors: De Gieter S, Eluru G, Schenck S, Stroobants A, Efremov RG, Brunner JD
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.
Eluru G, De Gieter S, Schenck S, Stroobants A, Shrestha B, Erbel P, Brunner JD, Efremov RG
(2025) Nat Methods
PUBMED: 41233542
DOI: doi:10.1038/s41592-025-02894-x
ISSN: 1548-7105