EMD-50715

Single-particle
2.66 Å
EMD-50715
3D View Gallery
Deposition: 18/06/2024
Map released: 17/12/2025
Last modified: 10/06/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-50715

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9frj summary report (pdf.gz) Map-9frj FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Ap4A bound in de novo transcription initiation T. thermophilus RNA polymerase complex with aTT DNA template

EMD-50715

Single-particle
2.66 Å
EMD-50715
3D View Gallery
Deposition: 18/06/2024
Map released: 17/12/2025
Last modified: 10/06/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Thermus thermophilus HB8, synthetic construct
Sample: heteropentamer 2:1:1:1 in complex with DNA duplex and nucleoside phosphates
Fitted models: 9frj

Deposition Authors: Skerlova J , Dubankova A, Svachova H , Kouba T
Molecular insight into 5' RNA capping with Np n Ns by bacterial RNA polymerase.
Serianni VM , Skerlova J , Dubankova AK, Skriba A , Svachova H , Vuckova T , Filimonenko A, Fabry M , Rezacova P , Kouba T , Cahova H
(2026) Nat Chem Biol , 22 , 917 - 924
PUBMED: 41513851
DOI: doi:10.1038/s41589-025-02134-5
ISSN: 1552-4469