EMD-50511

Single-particle
3.48 Å
EMD-50511
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Deposition: 31/05/2024
Map released: 02/04/2025
Last modified: 07/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-50511
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9fjs summary report (pdf.gz) Map and 9fjs FULL report (pdf.gz)
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Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - sigma-B udocked conformation

EMD-50511

Single-particle
3.48 Å
EMD-50511
3D View Gallery
Deposition: 31/05/2024
Map released: 02/04/2025
Last modified: 07/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Mycobacterium tuberculosis H37Rv, Escherichia coli
Sample: RNA polymerase holoenzyme bound to -10 promoter element ssDNA oligo
Fitted models: 9fjs

Deposition Authors: Brodolin K , Blaise M
Single-stranded DNA drives sigma subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations.
Vishwakarma RK, Marechal N, Morichaud Z, Blaise M, Margeat E, Brodolin K
(2025) Nucleic Acids Res , 53
PUBMED: 40240004
DOI: doi:10.1093/nar/gkaf272
ISSN: 1362-4962
ASTM: NARHAD