EMD-49174

Single-particle
3.42 Å
EMD-49174
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 04/03/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-49174
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9n9o summary report (pdf.gz) Map and 9n9o FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the dCas12f-gRNA-dsDNA complex (full R-Loop)

EMD-49174

Single-particle
3.42 Å
EMD-49174
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 04/03/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Flagellimonas taeanensis
Sample: dCas12f-gRNA-target DNA complex
Fitted models: 9n9o

Deposition Authors: Chang L , Sternberg SH, Xiao R , Hoffmann FT, Wiegand T, Xie D
Structural basis of RNA-guided transcription by a dCas12f-SigmaE-RNAP complex
Renjian X, Hoffmann FT, Xie D, Wiegand T, Palmieri AI, Sternberg SH, Leifu C
(2026) Nature
DOI: doi:10.1038/s41586-026-10178-3
ISSN: 1476-4687
ASTM: NATUAS