EMD-49174

Single-particle
3.42 Å
EMD-49174
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 20/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-49174

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9n9o summary report (pdf.gz) Map-9n9o FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of the dCas12f-gRNA-dsDNA complex (full R-Loop)

EMD-49174

Single-particle
3.42 Å
EMD-49174
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 20/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Flagellimonas taeanensis
Sample: dCas12f-gRNA-target DNA complex
Fitted models: 9n9o

Deposition Authors: Chang L , Sternberg SH , Xiao R , Hoffmann FT , Wiegand T , Xie D
Structural basis of RNA-guided transcription by a dCas12f-sigma E -RNAP complex.
Xiao R , Hoffmann FT , Xie D, Wiegand T , Palmieri AI, Sternberg SH , Chang L
(2026) Nature , 653 , 288 - 296
PUBMED: 41781609
DOI: doi:10.1038/s41586-026-10178-3
ISSN: 1476-4687
ASTM: NATUAS