EMD-49173

Single-particle
3.38 Å
EMD-49173
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 18/03/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-49173
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9n9m summary report (pdf.gz) Map and 9n9m FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the dCas12f-gRNA-DNA complex (partial R-Loop)

EMD-49173

Single-particle
3.38 Å
EMD-49173
3D View Gallery
Deposition: 11/02/2025
Map released: 24/12/2025
Last modified: 18/03/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Flagellimonas taeanensis
Sample: dCas12f-gRNA-DNA complex
Fitted models: 9n9m

Deposition Authors: Chang L , Sternberg SH , Xiao R , Hoffmann FT , Wiegand T , Xie D
Structural basis of RNA-guided transcription by a dCas12f-sigma E -RNAP complex.
Xiao R , Hoffmann FT , Xie D, Wiegand T , Palmieri AI, Sternberg SH , Chang L
(2026) Nature
PUBMED: 41781609
DOI: doi:10.1038/s41586-026-10178-3
ISSN: 1476-4687
ASTM: NATUAS