EMD-47941

Single-particle
2.95 Å
EMD-47941
3D View Gallery
Deposition: 16/11/2024
Map released: 18/06/2025
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-47941

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9ed9 summary report (pdf.gz) Map-9ed9 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint)

EMD-47941

Single-particle
2.95 Å
EMD-47941
3D View Gallery
Deposition: 16/11/2024
Map released: 18/06/2025
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Ternary complex - SpCas9, mm2 sgRNA, 55-bp dsDNA
Fitted models: 9ed9

Deposition Authors: Kiernan KA , Taylor DW
Visualization of a multi-turnover Cas9 after product release.
Kiernan KA , Taylor DW
(2025) Nat Commun , 16 , 5681 - 5681
PUBMED: 40593576
DOI: doi:10.1038/s41467-025-60668-7
ISSN: 2041-1723