EMD-45631

Single-particle
3.6 Å
EMD-45631
3D View Gallery
Deposition: 06/07/2024
Map released: 08/01/2025
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-45631

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9cjh summary report (pdf.gz) Map-9cjh FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 1

EMD-45631

Single-particle
3.6 Å
EMD-45631
3D View Gallery
Deposition: 06/07/2024
Map released: 08/01/2025
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Acidaminococcus sp. BV3L6
Sample: Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 1
Fitted models: 9cjh

Deposition Authors: Soczek KM, Doudna JA
CRISPR-Cas12a bends DNA to destabilize base pairs during target interrogation.
Soczek KM, Cofsky JC, Tuck OT, Shi H , Doudna JA
(2025) Nucleic Acids Res , 53
PUBMED: 39698811
DOI: doi:10.1093/nar/gkae1192
ISSN: 1362-4962
ASTM: NARHAD