EMD-40922

Single-particle
3.63 Å
EMD-40922
3D View Gallery
Deposition: 30/05/2023
Map released: 02/04/2025
Last modified: 30/04/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40922

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8szw summary report (pdf.gz) Map-8szw FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Reconstituted E. coli RNA polymerase post-termination complex on negatively-supercoiled DNA: open duplex DNA (rPTCo)

EMD-40922

Single-particle
3.63 Å
EMD-40922
3D View Gallery
Deposition: 30/05/2023
Map released: 02/04/2025
Last modified: 30/04/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, synthetic construct
Sample: E. coli Post-termination complex with open DNA bubble on negatively supercoiled DNA
Fitted models: 8szw

Deposition Authors: Brewer JJ, Darst SA , Campbell EA
RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation.
Brewer JJ, Inlow K , Mooney RA, Bosch B, Olinares PDB, Marcelino LP , Chait BT , Landick R , Gelles J , Campbell EA , Darst SA
(2025) Nat Struct Mol Biol , 32 , 639 - 649
PUBMED: 39779919
DOI: doi:10.1038/s41594-024-01447-8
ISSN: 1545-9985