EMD-40448

Single-particle
3.5 Å
EMD-40448
3D View Gallery
Deposition: 11/04/2023
Map released: 03/07/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40448
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8sfq summary report (pdf.gz) Map and 8sfq FULL report (pdf.gz)
EMICSS annotations (xml)

WT CRISPR-Cas12a post nontarget strand-cleavage with the the RuvC active site exposed.

EMD-40448

Single-particle
3.5 Å
EMD-40448
3D View Gallery
Deposition: 11/04/2023
Map released: 03/07/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Acidaminococcus sp. BV3L6
Sample: WT AsCas12a incubated with 20bp-complementary target DNA with phosphorothioate-modified target strand.
Fitted models: 8sfq

Deposition Authors: Strohkendl I, Taylor DW
Cas12a domain flexibility guides R-loop formation and forces RuvC resetting.
Strohkendl I, Saha A , Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
(2024) Mol Cell , 84 , 2717 - 2731.e6
PUBMED: 38955179
DOI: doi:10.1016/j.molcel.2024.06.007
ISSN: 1097-2765
ASTM: MOCEFL