EMD-40445

Single-particle
3.3 Å
EMD-40445
3D View Gallery
Deposition: 11/04/2023
Map released: 03/07/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40445

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8sfn summary report (pdf.gz) Map-8sfn FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

WT CRISPR-Cas12a with a 16bp R-loop and nontarget strand in the RuvC active site.

EMD-40445

Single-particle
3.3 Å
EMD-40445
3D View Gallery
Deposition: 11/04/2023
Map released: 03/07/2024
Last modified: 22/01/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Acidaminococcus sp. BV3L6
Sample: WT AsCas12a incubated with 16bp-complementary target DNA.
Fitted models: 8sfn

Deposition Authors: Strohkendl I, Taylor DW
Cas12a domain flexibility guides R-loop formation and forces RuvC resetting.
Strohkendl I, Saha A , Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
(2024) Mol Cell , 84 , 2717 - 2731.e6
PUBMED: 38955179
DOI: doi:10.1016/j.molcel.2024.06.007
ISSN: 1097-2765
ASTM: MOCEFL