EMD-3320

Single-particle
10.2 Å
EMD-3320
3D View Gallery
Deposition: 01/02/2016
Map released: 24/02/2016
Last modified: 08/02/2017
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-3320
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

CryoEM structure of the ATP-treated CMG replicative helicase (compact state)

EMD-3320

Single-particle
10.2 Å
EMD-3320
3D View Gallery
Deposition: 01/02/2016
Map released: 24/02/2016
Last modified: 08/02/2017
Overview 3D View Sample Experiment Validation Additional data Links
Name: CMG treated with ATP
Sample [1]
Name: CMG treated with ATP
Number of unique components: 11
Molecular weight
Experimental Theoretical Method
700 kDa 700 kDa -
Protein [11]
Recombinant expression
Name: Mcm2
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
100 kDa 100 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Trichoplusia ni - High Five pFastBac-Mcm2
Recombinant expression
Name: Mcm3
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
90 kDa 90 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Mcm3
Recombinant expression
Name: Mcm4
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
97 kDa 97 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Mcm4
Recombinant expression
Name: Mcm5
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
82 kDa 82 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Mcm5
Recombinant expression
Name: Mcm6
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
92 kDa 92 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Mcm6
Recombinant expression
Name: Mcm7
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
81 kDa 81 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Mcm7
Recombinant expression
Name: Cdc45
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
66 kDa 66 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Cdc45
Recombinant expression
Name: Psf1
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
23 kDa 23 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Psf1
Recombinant expression
Name: Psf2
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
23 kDa 23 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Psf2
Recombinant expression
Name: Psf3
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
25 kDa 25 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Psf3
Recombinant expression
Name: Sld5
Number of copies: 1
Oligomeric state: Monomer
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Drosophila melanogaster (Fruit fly)
Molecular weight
Experimental Theoretical Method
26 kDa 26 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
unidentified baculovirus - - pFastBac-Sld5