EMD-24819

Single-particle
3.3 Å
EMD-24819
3D View Gallery
Deposition: 04/09/2021
Map released: 20/04/2022
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-24819

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-7s38 summary report (pdf.gz) Map-7s38 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cas9:sgRNA:DNA (S. pyogenes) forming a 3-base-pair R-loop

EMD-24819

Single-particle
3.3 Å
EMD-24819
3D View Gallery
Deposition: 04/09/2021
Map released: 20/04/2022
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Streptococcus pyogenes serotype M1, synthetic construct, Streptococcus pyogenes
Sample: Cas9 bound to sgRNA and DNA with 3bp complementarity between RNA and DNA
Fitted models: 7s38

Deposition Authors: Cofsky JC , Soczek KM
CRISPR-Cas9 bends and twists DNA to read its sequence.
Cofsky JC , Soczek KM , Knott GJ , Nogales E , Doudna JA
(2022) Nat Struct Mol Biol , 29 , 395 - 402
PUBMED: 35422516
DOI: doi:10.1038/s41594-022-00756-0
ISSN: 1545-9985