Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-22161

Single-particle
3.45 Å
EMD-22161
3D View Gallery
Deposition: 15/06/2020
Map released: 02/09/2020
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-22161
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6xf5 summary report (pdf.gz) Map and 6xf5 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of a biotinylated SARS-CoV-2 spike probe in the prefusion state (RBDs down)

EMD-22161

Single-particle
3.45 Å
EMD-22161
3D View Gallery
Deposition: 15/06/2020
Map released: 02/09/2020
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Severe acute respiratory syndrome coronavirus 2
Sample: SARS-CoV-2 spike ectodomain biotinylated probe in the prefusion state
Fitted models: 6xf5

Deposition Authors: Cerutti G, Gorman J
Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.
Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Acevedo SNL, Madan B, de Souza MO, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang L, Abiona O, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L, Kwong PD
(2020) SSRN , 3639618 - 3639618
PUBMED: 32742241
DOI: doi:10.2139/ssrn.3639618
ISSN: 1556-5068