EMD-1941

Subtomogram averaging
39.18 Å
EMD-1941
3D View Gallery
Deposition: 30/07/2011
Map released: 20/10/2011
Last modified: 29/08/2012
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-1941
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Radial Spoke from Chlamydomonas flagella

EMD-1941

Subtomogram averaging
39.18 Å
EMD-1941
3D View Gallery
Deposition: 30/07/2011
Map released: 20/10/2011
Last modified: 29/08/2012
Overview 3D View Sample Experiment Validation Additional data Links
Method: Subtomogram averaging
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.4
Details: HMDKP
Grid
Details: Lacey carbon grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 100 K
Instrument: OTHER
Method: 3 second blotting
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 1.5 mm
Nominal defocus: 3.0 µm - 6.0 µm
Nominal magnification: 27500.0
Calibrated magnification: 19000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626
Temperature
Minimum: 100 K
Average: 100 K
Maximum: 100 K
Specialist optics
Energy filter
Name: GIF
Lower energy threshold: 0.0 eV
Upper energy threshold: 20.0 eV
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 1000 (2k x 2k)
Average electron dose per image: 0.6 e/Å2
Tilt Series [1]
Axis 1 Axis 2
Min. Max. Inc. Min. Max. Inc. Rotation
-60 ° 60 ° - - - - -
Image processing [1]
Details: Subtomograms were averaged. Average number of tilts used in the 3D reconstructions: 61. Average tomographic tilt angle increment: 2.
Final reconstruction
Resolution: 39.18 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Details:
Software [1]
Name Version Details
IMOD - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This is a density map of Radial Spokes from Chlamydomonas flagella.
Details: ::::EMDATABANK.org::::EMD-1941::::
⬡ Geometry
X Y Z
Origin 14 19 13
Dimensions (px) 89 43 143
Dimensions (Å) 603.42 291.54 969.54
Voxel size (Å) 6.78 6.78 6.78
Contour list
Primary Level Source
True 2.5 AUTHOR