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author:"Rustandi RR"

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Current Database

  • EMDB [5]
  • EMPIAR [1]

Current status

  • REL [6]

Sample type

  • Complex [5]
  • Ligand [5]
  • Protein [5]

Organism

  • Clostridioides difficile [5]
  • Clostridioides difficile [3]

EM Method

  • Single-particle [5]

Resolution

  • ≥ 2Å and < 3Å [1]
  • ≥ 3Å and < 4Å [4]

Model molecular weight

  • 500-1000 kDa [3]
  • 1-2 MDa [2]

Release Date

  • 2023 [4]
  • 2020 [2]

Author by name

  • Pozharski E [6]
  • Adipietro KA [5]
  • Rustandi RR [5]
  • Varney KM [5]
  • Weber DJ [5]
  • Abeyawardhane DL [3]
  • Ben-hail D [3]
  • Ruiz RG [3]
  • Silin VI [3]
  • Xu X [3]
  • Beckett D [2]
  • Christanti S [2]
  • Cleveland T [2]
  • Cook ME [2]
  • Georges AD [2]
  • Godoy-ruiz R [2]
  • Grishaev A [2]
  • Kristopeit A [2]
  • Lancaster C [2]
  • Loughney JW [2]
  • Mackerell AD [2]
  • Michel SLJ [2]
  • Neu HM [2]
  • Olson JW [2]
  • Peralta C [2]
  • Roth BM [2]
  • Wilder PT [2]
  • Yu W [2]
  • Des Georges A [1]

Author by ORCID

  • Yu W [2]
  • Cook ME [2]
  • Michel SLJ [2]
  • Ben-hail D [2]
  • Weber DJ [2]
  • Xu X [2]
  • Cleveland T [2]
  • Godoy-ruiz R [2]
  • Peralta C [2]

Journal

  • PNAS [3]
  • TO BE PUBLISHED [3]

Software

  • CRYOSPARC [3]

Microscope

  • TFS GLACIOS [3]
  • FEI TITAN KRIOS [2]

Electron Source

  • FIELD EMISSION GUN [5]

Film or detector model

  • GATAN K3 6K X 4K [3]
  • GATAN K2 SUMMIT 4K X 4K [2]

EMPIAR Experiment type

  • EMDB [1]

EMPIAR Image category

  • MICROGRAPHS - MULTIFRAME [1]
  • MICROGRAPHS - SINGLE FRAME [1]

External links

  • AlphaFold DB [5]
  • ChEBI [5]
  • DrugBank [5]
  • PDB [5]
  • PDBe-Kb [5]
  • UniProt [5]
  • EMPIAR [2]
  • Gene Ontology [2]
  • InterPro [2]
  • Pfam [2]
  • EMDB [1]
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EMPIAR-11703
Related EMDB
EMD-20926 EMD-20927
Clostridium difficile binary toxin translocase CDTb tetradecamer in symmetric and asymmetric conformations
Release date: Dec. 4, 2023
Dataset size: 1.8 TB
Experiment type: EMDB

Xu X, Godoy-Ruiz R, Adipietro KA, Peralta C, Ben-Hail D, Varney KM, Cook ME, Roth BM, Wilder PT, Cleveland T, Grishaev A, Neu HM, Michel SLJ, Yu W, Beckett D, Rustandi RR, Lancaster C, Loughney JW, Kristopeit A, Christanti S, Olson JW, MacKerell AD, Georges AD, Pozharski E, Weber DJ

Proc Natl Acad Sci U S A (2020) 117 pp. 1049-1058 [ DOI: 10.1073/pnas.1919490117 Pubmed: 31896582 ]

Image sets:
  • motion-corrected micrographs of CDTB (DW)
  • multi-frame micrographs of CDTB
  • motion-corrected micrographs of CDTB (no DW)
EMD-28205
Download Map
3D View
Clostridioides difficile binary toxin translocase CDTb wild-type after calcium depletion from receptor binding domain 1 RBD1 - Class 2
Release date: Sept. 27, 2023
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8ekk
Q-score: 0.538

Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ

To Be Published

EMD-28206
Download Map
3D View
Clostridioides difficile binary toxin translocase CDTb wild-type after calcium depletion from receptor binding domain 1 RBD1 - Class 1
Release date: Sept. 27, 2023
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8ekl
Q-score: 0.577

Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ

To Be Published

EMD-28207
Download Map
3D View
Clostridioides difficile binary toxin translocase CDTb double mutant - D623A D734A
Release date: Sept. 27, 2023
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8ekm
Q-score: 0.502

Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ

To Be Published

EMD-20926
Download Map
3D View
Raw data
EMPIAR-11703
Clostridium difficile binary toxin translocase CDTb in asymmetric tetradecamer conformation
Release date: Jan. 22, 2020
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6uwr
Q-score: 0.594

Xu X, Godoy-Ruiz R, Adipietro KA, Peralta C, Ben-Hail D, Varney KM, Cook ME, Roth BM, Wilder PT, Cleveland T, Grishaev A, Neu HM, Michel SLJ, Yu W, Beckett D, Rustandi RR, Lancaster C, Loughney JW, Kristopeit A, Christanti S, Olson JW, MacKerell AD, Georges AD, Pozharski E, Weber DJ

PNAS (2020) 117 pp. 1049-1058 [ Pubmed: 31896582 DOI: doi:10.1073/pnas.1919490117 ]

EMD-20927
Download Map
3D View
Raw data
EMPIAR-11703
Clostridium difficile binary toxin translocase CDTb tetradecamer in symmetric conformation
Release date: Jan. 22, 2020
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6uwt
Q-score: 0.472

Xu X, Godoy-Ruiz R, Adipietro KA, Peralta C, Ben-Hail D, Varney KM, Cook ME, Roth BM, Wilder PT, Cleveland T, Grishaev A, Neu HM, Michel SLJ, Yu W, Beckett D, Rustandi RR, Lancaster C, Loughney JW, Kristopeit A, Christanti S, Olson JW, MacKerell AD, Georges AD, Pozharski E, Weber DJ

PNAS (2020) 117 pp. 1049-1058 [ Pubmed: 31896582 DOI: doi:10.1073/pnas.1919490117 ]

Sort by: Download
Showing 1 - 6 of 6. Page 1 of 1
Display:
Statistics
×

Download












































Preview first 10
Download

Filter By

Current Database

  • EMDB [5]
  • EMPIAR [1]

Current status

  • REL [6]

Sample type

  • Complex [5]
  • Ligand [5]
  • Protein [5]

Organism

  • Clostridioides difficile [5]
  • Clostridioides difficile [3]

EM Method

  • Single-particle [5]

Resolution

  • ≥ 2Å and < 3Å [1]
  • ≥ 3Å and < 4Å [4]

Model molecular weight

  • 500-1000 kDa [3]
  • 1-2 MDa [2]

Release Date

  • 2023 [4]
  • 2020 [2]

Author by name

  • Pozharski E [6]
  • Adipietro KA [5]
  • Rustandi RR [5]
  • Varney KM [5]
  • Weber DJ [5]
  • Abeyawardhane DL [3]
  • Ben-hail D [3]
  • Ruiz RG [3]
  • Silin VI [3]
  • Xu X [3]
  • Beckett D [2]
  • Christanti S [2]
  • Cleveland T [2]
  • Cook ME [2]
  • Georges AD [2]
  • Godoy-ruiz R [2]
  • Grishaev A [2]
  • Kristopeit A [2]
  • Lancaster C [2]
  • Loughney JW [2]
  • Mackerell AD [2]
  • Michel SLJ [2]
  • Neu HM [2]
  • Olson JW [2]
  • Peralta C [2]
  • Roth BM [2]
  • Wilder PT [2]
  • Yu W [2]
  • Des Georges A [1]

Author by ORCID

  • Yu W [2]
  • Cook ME [2]
  • Michel SLJ [2]
  • Ben-hail D [2]
  • Weber DJ [2]
  • Xu X [2]
  • Cleveland T [2]
  • Godoy-ruiz R [2]
  • Peralta C [2]

Journal

  • PNAS [3]
  • TO BE PUBLISHED [3]

Software

  • CRYOSPARC [3]

Microscope

  • TFS GLACIOS [3]
  • FEI TITAN KRIOS [2]

Electron Source

  • FIELD EMISSION GUN [5]

Film or detector model

  • GATAN K3 6K X 4K [3]
  • GATAN K2 SUMMIT 4K X 4K [2]

EMPIAR Experiment type

  • EMDB [1]

EMPIAR Image category

  • MICROGRAPHS - MULTIFRAME [1]
  • MICROGRAPHS - SINGLE FRAME [1]

External links

  • AlphaFold DB [5]
  • ChEBI [5]
  • DrugBank [5]
  • PDB [5]
  • PDBe-Kb [5]
  • UniProt [5]
  • EMPIAR [2]
  • Gene Ontology [2]
  • InterPro [2]
  • Pfam [2]
  • EMDB [1]