EMD-9763

Single-particle
3.8 Å
EMD-9763
3D View Gallery
Deposition: 24/12/2018
Map released: 10/04/2019
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-9763
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6j0f summary report (pdf.gz) Map and 6j0f FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath/tube terminator in extended state

EMD-9763

Single-particle
3.8 Å
EMD-9763
3D View Gallery
Deposition: 24/12/2018
Map released: 10/04/2019
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Helical Array
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 46.4 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 45000
⌯ Applied Symmetry
Point group: C6
Startup model [1]
Type: NONE
⦨ Initial angle assignment
Type: NOT APPLICABLE
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 256 256 256
Dimensions (Å) 286.976 286.976 286.976
Voxel size (Å) 1.121 1.121 1.121
Contour list
Primary Level Source
True 0.14 AUTHOR