EMD-9336

Single-particle
3.9 Å
EMD-9336
3D View Gallery
Deposition: 14/11/2018
Map released: 20/02/2019
Last modified: 20/02/2019
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-9336
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Bacillus PS3 ATP synthase FO region class 1

EMD-9336

Single-particle
3.9 Å
EMD-9336
3D View Gallery
Deposition: 14/11/2018
Map released: 20/02/2019
Last modified: 20/02/2019
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 10 mg/mL
Buffer
pH: 7.4
Grid
Details: Unspecified
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 100%
Chamber temperature: 4 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 75000.0
Calibrated magnification: 132075.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Dimensions: 4096 pixel x 4096 pixel
Average exposure time: 60.0 s
Average electron dose per image: 0.71 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 405432
Algorithm: BACK PROJECTION
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2.1 -
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
CryoSPARC 2.0 -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION 2.1 -
Particle selection [1]
Selected Ref. model Method Software Details
1238140 - - - -
Final 3D classification
Number of classes: 4
Software [1]
Name Version Details
CryoSPARC 2.0 -
CTF correction
Software [1]
Name Version Details
CTFFIND 4.1 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: ATP synthase FO region class 1
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 320 320 320
Dimensions (Å) 339.19998 339.19998 339.19998
Voxel size (Å) 1.06 1.06 1.06
Contour list
Primary Level Source
True 0.032 AUTHOR