EMD-8826

Single-particle
3.6 Å
EMD-8826
3D View Gallery
Deposition: 11/07/2017
Map released: 02/05/2018
Last modified: 01/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8826

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-5wf0 summary report (pdf.gz) Map-5wf0 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex I)

EMD-8826

Single-particle
3.6 Å
EMD-8826
3D View Gallery
Deposition: 11/07/2017
Map released: 02/05/2018
Last modified: 01/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, Escherichia coli O139:H28 (strain E24377A / ETEC)
Sample: 70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex 1)
Fitted models: 5wf0

Deposition Authors: Fislage M , Frank J
Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
Fislage M , Zhang J, Brown ZP , Mandava CS , Sanyal S , Ehrenberg M, Frank J
(2018) Nucleic Acids Res , 46 , 5861 - 5874
PUBMED: 29733411
DOI: doi:10.1093/nar/gky346
ISSN: 1362-4962
ASTM: NARHAD