EMD-8770

Single-particle
25.0 Å
EMD-8770
3D View Gallery
Deposition: 15/06/2017
Map released: 19/07/2017
Last modified: 01/11/2017
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8770
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Negative stain of influenza B virus recombinant neuraminidase

EMD-8770

Single-particle
25.0 Å
EMD-8770
3D View Gallery
Deposition: 15/06/2017
Map released: 19/07/2017
Last modified: 01/11/2017
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.3
Staining
Type: NEGATIVE
Material: Uranyl formate
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Image Recording [1]
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Average electron dose per image: 100.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 47592
Software [1]
Name Version Details
RELION 1.4 -
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Influenza B virus neuraminidase
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 200 200 200
Dimensions (Å) 360.0 360.0 360.0
Voxel size (Å) 1.8 1.8 1.8
Contour list
Primary Level Source
True 0.272 EMDB