EMD-8681

Single-particle
7.9 Å
EMD-8681
3D View Gallery
Deposition: 13/04/2017
Map released: 23/08/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8681
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5vhp summary report (pdf.gz) Map and 5vhp FULL report (pdf.gz)
EMICSS annotations (xml)

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle

EMD-8681

Single-particle
7.9 Å
EMD-8681
3D View Gallery
Deposition: 13/04/2017
Map released: 23/08/2017
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TECNAI ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 7.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 11767
Startup model [1]
Type: INSILICO MODEL
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Final Map of Rpn1-p28-AAA subcomplex in the TA5 state, corrected with a B-factor of -450
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 360 360 360
Dimensions (Å) 309.6 309.6 309.6
Voxel size (Å) 0.86 0.86 0.86
Contour list
Primary Level Source
True 0.004 AUTHOR