EMD-8585

Single-particle
4.05 Å
EMD-8585
3D View Gallery
Deposition: 02/02/2017
Map released: 05/04/2017
Last modified: 16/08/2017
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8585
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5upa summary report (pdf.gz) Map and 5upa FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM structure of crosslinked E.coli RNA polymerase elongation complex

EMD-8585

Single-particle
4.05 Å
EMD-8585
3D View Gallery
Deposition: 02/02/2017
Map released: 05/04/2017
Last modified: 16/08/2017
Overview 3D View Sample Experiment Validation Additional data Links
Name: Crosslinked E.coli RNA polymerase ternary elongation complex
Complex [1]
Name: Crosslinked E.coli RNA polymerase ternary elongation complex
Natural source [1]
Organism: Escherichia coli
Recombinant Expression [1]
Organism Strain Cell Plasmid
Escherichia coli - - -
Molecular weight
Experimental Theoretical Method
- 393 kDa -
DNA [2]
DNA
Name: DNA (29-MER)
Number of copies: 1
Natural source
Organism: T7likevirus
Molecular weight
Experimental Theoretical Method
- 8 kDa -
DNA
Name: DNA (29-MER)
Number of copies: 1
Details: Template DNA
Natural source
Organism: T7likevirus
Molecular weight
Experimental Theoretical Method
- 8 kDa -
RNA [1]
Name: RNA (5'-R(*GP*CP*A*UP*UP*CP*AP*AP*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')
Number of copies: 1
Natural source
Organism: T7likevirus
Molecular weight
Experimental Theoretical Method
- 6 kDa -
Protein [4]
LEVO
Name: DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Number of copies: 2
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Escherichia coli O157:H7
Molecular weight
Experimental Theoretical Method
- 26 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Number of copies: 1
UniProtKB AlphaFold DB
Natural source
Organism: Escherichia coli O45:K1 (strain S88 / ExPEC)
Strain: S88 / ExPEC
Molecular weight
Experimental Theoretical Method
- 150 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Escherichia coli O157:H7
Molecular weight
Experimental Theoretical Method
- 155 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: DNA-directed RNA polymerase subunit omega
EC number: 2.7.7.6
Number of copies: 1
UniProtKB AlphaFold DB
Natural source
Organism: Escherichia coli O45:K1 (strain S88 / ExPEC)
Strain: S88 / ExPEC
Molecular weight
Experimental Theoretical Method
- 9 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
Ligand [2]
Name: MAGNESIUM ION
HET code: MG
Number of copies: 1
ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 24 Da -
Name: ZINC ION
HET code: ZN
Number of copies: 2
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -