EMD-8553

Single-particle
6.7 Å
EMD-8553
3D View Gallery
Deposition: 13/01/2017
Map released: 12/04/2017
Last modified: 29/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8553
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Vps4-Vta1 complex, VSL_B

EMD-8553

Single-particle
6.7 Å
EMD-8553
3D View Gallery
Deposition: 13/01/2017
Map released: 12/04/2017
Last modified: 29/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Grid
Material: COPPER
Vitrification
Cryogen name: ETHANE
Chamber humidity: 80%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK III
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average exposure time: 0.2 s
Average electron dose per image: 1.3 e/Å2
Image processing [1]
Final reconstruction
Resolution: 6.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 38421
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: INSILICO MODEL
Details:E2initialmodel.py
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Vps4-Vta1 complex, VSL_B
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 138 138 138
Dimensions (Å) 194.442 194.442 194.442
Voxel size (Å) 1.409 1.409 1.409
Contour list
Primary Level Source
True 0.0253 AUTHOR