EMD-8544

Subtomogram averaging
17.0 Å
EMD-8544
3D View Gallery
Deposition: 04/01/2017
Map released: 29/03/2017
Last modified: 29/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8544
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Salmonella Type III secretion injectisome

EMD-8544

Subtomogram averaging
17.0 Å
EMD-8544
3D View Gallery
Deposition: 04/01/2017
Map released: 29/03/2017
Last modified: 29/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Method: Subtomogram averaging
Aggregation State: Cell
Specimen preparation [1]
Buffer
pH: 7.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: OTHER
Imaging mode: DARK FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 1.4 e/Å2
Image processing [1]
Final reconstruction
Resolution: 17.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
⌯ Applied Symmetry
Point group: C6
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
I3 - -
Extraction
Number of images used: 5274
Software [1]
Name Version Details
IMOD - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Salmonella injectisome
⬡ Geometry
X Y Z
Origin -200 -200 -200
Dimensions (px) 400 400 400
Dimensions (Å) 1040.0 1040.0 1040.0
Voxel size (Å) 2.6 2.6 2.6
Contour list
Primary Level Source
True 2.53 AUTHOR